Increased long noncoding RNA SNHG20 predicts poor prognosis in colorectal cancer

BMC Cancer

Research Article

Mao-Ming Xiong¹  Shao-Wen Zhu²  Cong Li²  Li Zhou²  Xue-Qing Fang²  Jun He² 

[1] Department of General Surgery, First Hospital Affiliated to Anhui Medical University, 230022, Hefei, China;Department of Minimally Invasive Surgery, The People’s Hospital of Chizhou, 247000, Chizhou, China;

关键词: Long noncoding RNA;    SNHG20;    Colorectal cancer;    Cell cycle;   

DOI  :  10.1186/s12885-016-2719-x

 received in 2016-05-28, accepted in 2016-08-11,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundLong noncoding RNAs (lncRNAs) have been suggested to be involved in the development and progression of malignancies. However, the investigation of small nucleolar RNA host gene 20 (SNHG20) on cancer progression remains unknown. The present study aims to explore the clinical significance of SNHG20 and its potential molecular mechanism in colorectal cancer (CRC).MethodsQuantitative real-time PCR (qRT-PCR) was used to measure the SNHG20 expression in a total of 107 CRC tissues and CRC cell lines. Loss of function approach was employed to explore the biological roles of SNHG20 in vitro. Its potential molecular mechanism was further verified by western blotting and qRT-PCR.ResultsThe results suggested that SNHG20 expression was significantly upregulated in CRC tissues compared to corresponding normal tissues from 107 CRC patients. High expression of SNHG20 was remarkably associated with advanced TNM stage in patients with CRC. Multivariate analyses unraveled that SNHG20 expression was an independent prognostic factor for overall survival in CRC patients. Further functional assays revealed that knockdown of SNHG20 suppressed cell proliferation, invasion and migration, and cell cycle progression in CRC cells. Moreover, SNHG20 regulated cell growth through modulation of a series of cell cycle-associated genes.ConclusionsOur findings suggest that dysregulation of SNHG20 participates in CRC progression and may serve as a potential therapeutic target in CRC patients.

【 授权许可】

CC BY   
© The Author(s). 2016