Application of ellipsometry, spr-technic and raman-spectroscopy into diagnosis of colorectal cancer

3rd International Conference "Cognitive Robotics"

计算机科学

Kruchinina, M.V.^1^2^5 ; Gromov, A.A.^1 ; Prudnikova, Y.I.^1 ; Generalov, V.M.^3^5 ; Kruchinin, V.N.^4^5 ; Volodin, V.A.^4 ; Shuvalov, G.V.^5 ; Minin, O.V.^6 ; Shehovtsov, S.V.^1 ; Peltec, S.E.^1 ; Minin, I.V.^5^6

Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia^1

Novosibirsk State Medical University, Novosibirsk, Russia^2

Science State Research Center of Virology and Biotechnology Vector, Novosibirsk Region, Koltsovo, Russia^3

Rzhanov Institute of Semiconductor Physics, Novosibirsk, Russia^4

Siberian State Order of the Red Banner of Labor Institute of Metrology, Novosibirsk, Russia^5

Tomsk State University, Tomsk, Russia^6

关键词: Colorectal cancer;    Colorectal carcinoma;    Effective thickness;    Predictive accuracy;    Scanning ellipsometry;    Specific interaction;    SPR (surface plasmon resonance);    Velocity constants;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/516/1/012017/pdf
DOI  :  10.1088/1757-899X/516/1/012017

学科分类：计算机科学（综合）

来源: IOP

【 摘 要 】

Experiments with application of the "Ellips-SPEK" spectral ellipsometric SPR (Surface Plasmon Resonance) analyzer, validating the results on the basis of ProteOn XPR36, revealed the differences in the values of velocity constants of specific interactions of monoclonal highly specific antibodies with tumor M2-pyruvate kinase on the surface of blood serum biochips in patients with various stages colorectal carcinoma (CRC) and location of metastases (AUC 0.89). Scanning ellipsometry enabled to reveal a significant increase of biomolecule surface effective thickness resulting from the specific "antigen-antibody" interaction in the group of patients with extrahepatic and hepatic metastases, as compared to the control group with healthy patients (p-1 in the same groups of patients (p