The effects of dopamine receptor 2 expression on B cells on bone metabolism and TNF-α levels in rheumatoid arthritis

BMC Musculoskeletal Disorders

Research Article

Chi Zhang¹  Tao Yue²  Qi Zhu²  Dong-Yi He²  Xiu-Fang Kong³  Ying Sun³  Lei Wei³  Lin-Di Jiang⁴ 

[1] Department of Orthopedics, Zhongshan Hospital, Fudan University, Shanghai, China;Department of Rheumatology, Guanghua Integrative Medicine Hospital, Shanghai, China;Department of Rheumatology, Zhongshan Hospital, Fudan University, No. 180, Road Fenglin, 200032, Shanghai, People’s Republic of China;Department of Rheumatology, Zhongshan Hospital, Fudan University, No. 180, Road Fenglin, 200032, Shanghai, People’s Republic of China;Center of evidence based medicine, Fudan University, Shanghai, China;

关键词: B cells;    Dopamine receptor;    Rheumatoid arthritis;    Bone metabolism;   

DOI  :  10.1186/s12891-016-1220-7

 received in 2015-12-17, accepted in 2016-08-13,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundDopamine receptor 2 (DR2) expressions on B cells from Rheumatoid arthritis (RA) patients has been found to be negatively correlated with disease activity and can potentially predict the response to treatment. This study aimed to investigate the role of B cell DR2 expression on bone remodeling in RA.MethodsPatients with RA (n = 14) or osteoarthritis (OA; n = 12), and healthy controls (n = 12) were recruited for this study. Dopamine receptor (DR) 2 expression was assessed using flow cytometry. Pro-inflammatory cytokines, including interleuin(IL)-1β, IL-6, IL-17, and tumor necrosis factor(TNF)-α, and bone turnovers, including osteocalcin (OC),serum procollagen type I N propeptide (PINP), C-terminal telopeptide of type I collagen (β-CTX), collagen type I cross-linked telopeptide (ICTP), as well as matrix metalloproteinase-3 (MMP-3) and osteoprotegerin (OPG) were measured by electrochemiluminescence, chemiluminescence, or enzyme-linked immunosorbent assay. DR2 expression on synovial B cells from 4 RA patients and 3 OA patients was detected by immunofluorescence.ResultsThere were more DR2+CD19+ B cells in synovial tissues from RA patients than in those from OA patients. The frequency of peripheral B cells that expressed DR2 was positively correlated with plasma TNF-α level. Levels of ICTP and MMP-3 were significantly higher, and OPG were lower in RA patients compared to those in the OA group and healthy controls (all P < 0.05).ConclusionThe frequency of B cells that expressed DR2 showed a correlation with levels of the pro-inflammatory cytokine TNF-α. DR2+CD19+ B cells in synovial tissues might have a role in bone metabolism and TNF-α production.

【 授权许可】

CC BY   
© The Author(s). 2016