Competitive DNA transfection formulation via electroporation for human adipose stem cells and mesenchymal stem cells

Biological Procedures Online

Research

Michael Flanagan¹  Xueqing Xia²  Shulin Li³  Bruce A Bunnell⁴  Jeffrey M Gimble⁵  Gang Yu⁵ 

[1] Department of Comparative Biomedical Sciences, Louisiana State University, Skip Bertman Drive, 70803, Baton Rouge, LA, USA;Department of Pediatrics Research, The University Texas MD Anderson Cancer Center, Graduate School of Biomedical Sciences, 1515, Holcombe, Houston, TX, USA;Department of Pediatrics Research, The University Texas MD Anderson Cancer Center, Graduate School of Biomedical Sciences, 1515, Holcombe, Houston, TX, USA;Department of Pediatrics Research, The University of Texas MD Anderson Cancer Center, The University of Texas Graduate School of Biomedical Sciences at Houston, 77030, Houston, TX, USA;Director, Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, 1430 Tulane Ave, New Orleans, LA, USA;Pennington Biomedical Research Center, 6400 Perkins Rd., 70808, Baton Rouge, LA, USA;

关键词: Electroporation;    Formulation;    Stem cells;    Transfection;    Cell therapy;   

DOI  :  10.1186/1480-9222-14-7

 received in 2012-01-30, accepted in 2012-04-18,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundAdipose stem cells have a strong potential for use in cell-based therapy, but the current nucleofection technique, which relies on unknown buffers, prevents their use.ResultsWe developed an optimal nucleofection formulation for human adipose stem cells by using a three-step method that we had developed previously. This method was designed to determine the optimal formulation for nucleofection that was capable of meeting or surpassing the established commercial buffer (Amaxa), in particular for murine adipose stem cells. By using this same buffer, we determined that the same formulation yields optimal transfection efficiency in human mesenchymal stem cells.ConclusionsOur findings suggest that transfection efficiency in human stem cells can be boosted with proper formulation.

【 授权许可】

Unknown   
© Flanagan et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.