Plantamajoside from Plantago asiatica modulates human umbilical vein endothelial cell dysfunction by glyceraldehyde-induced AGEs via MAPK/NF-κB

BMC Complementary and Alternative Medicine

Research Article

Kwang-Won Lee¹  Won-rak Son¹  Mi-Hyun Nam²  Chung-Oui Hong³  Yoonsook Kim⁴ 

[1] Department of Biotechnology, College of Life Science and Biotechnology, Korea University, 02841, Seoul, South Korea;Department of Ophthalmology, University of Colorado School of Medicine, 80045, Aurora, CO, USA;International Ginseng & Herb Research Institute, 32724, Geumsan-gu, Chungnam, Republic of Korea;Korea Food Research Institute, 13539, Seongnam-si, Gyeonggi, South Korea;

关键词: Endothelial dysfunction;    Adhesion molecule;    Monocyte adhesion;    Advanced glycation end-products;    Plantamajoside;   

DOI  :  10.1186/s12906-017-1570-1

 received in 2016-08-22, accepted in 2017-01-07,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundPlantago asiatica has been traditionally used for traditional medicine around East Asia. Plantamajoside (PM), which is isolated from this plant, is known for biological properties including anti-inflammation and antioxidant activity. To demonstrate the biological activity of PM against endothelial dysfunction induced by advanced glycation end-products (AGEs), a cellular inflammatory mechanism system was evaluated in human umbilical vein endothelial cells (HUVECs).MethodsWe obtained PM through previous research in our laboratory. We formed the AGEs from bovine serum albumin with glyceraldehyde in the dark for seven days. To confirm the modulation of the inflammatory mechanism in endothelial dysfunction, we quantified the various pro-inflammatory cytokines and endothelial dysfunction-related proteins in the HUVECs with Western blotting and with real-time and quantitative real-time polymerase chain reactions.ResultsCo-treatment with PM and AGEs significantly suppressed inflammatory cytokines and adhesion molecule expression. Moreover, the PM treatment for down-regulated inflammatory signals and blocked monocyte adhesion on the HUVECs.ConclusionsTheses results demonstrated that PM, as a potential natural compound, protects AGE-induced endothelial cells against inflammatory cellular dysfunction.

【 授权许可】

CC BY   
© The Author(s). 2017