Effect of catalpol on remyelination through experimental autoimmune encephalomyelitis acting to promote Olig1 and Olig2 expressions in mice

BMC Complementary and Alternative Medicine

Research Article

Tao Yang¹  Yongping Fan¹  Su Wang¹  Hui Zhao²  Lei Liu²  Ling Fang²  Lei Wang²  Qi Zheng³ 

[1] Department of Traditional Chinese Medicine, Beijing Tiantan Hospital, Capital Medical University, 100050, Beijing, People’s Republic of China;School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, 100069, Beijing, People’s Republic of China;School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, 100069, Beijing, People’s Republic of China;Oncology Department, Guang An Men Hospital of China Academy of Chinese Medical Sciences, 100053, Beijing, People’s Republic of China;

关键词: Catalpol;    Experimental autoimmune encephalomyelitis;    Multiple sclerosis;    Remyelination;    Olig1;    Olig2;   

DOI  :  10.1186/s12906-017-1642-2

 received in 2015-10-23, accepted in 2017-02-21,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundMultiple sclerosis (MS) as an autoimmune disorder is a common disease occurring in central nervous system (CNS) and the remyelination plays a pivotal role in the alleviating neurological impairment in the MS. Catalpol, an effective component extracted from the Chinese herb Radix Rehmanniae, which has been proved protective in cerebral diseases.MethodsTo determine the protective effects and mechanisms of Catalpol on MS, the mice with experimental autoimmune encephalomyelitis (EAE) were induced by myelin oligodendrocyte glycoprotein (MOG) 35–55, as a model for human MS. Th17 cells were counted by flow cytometric (FCM). The expressions of nerve-glial antigen (NG) 2 and myelin basic protein (MBP) were measured by immunohistochemical staining. Olig1+ and Olig2+/BrdU+ cells were counted by immunofluorescence. Olig1 and Olig2 gene expressions were detected by real-time fluorescent quantitative reverse transcription (qRT) -PCR.ResultsThe results showed that Catalpol improved neurological function, reduced inflammatory cell infiltration and demyelination. It could decrease Th17 cells in the peripheral blood. It increased the protein expressions of NG2 and MBP in mice brains, up-regulated markedly protein and gene expressions of Olig1 and Olig2 in terms of timing, site and targets.ConclusionsThese data demonstrated that Catalpol had a strong neuroprotective effect on EAE mice. Catalpol also plays a role in remyelination by promoting the expressions of Olig1 and Olig2 transcription factors.

【 授权许可】

CC BY   
© The Author(s). 2017

【 图 表 】

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