Effects of insulin-like growth factor 1 on pathologic processes in the cuprizone model of multiple sclerosis

3rd International Conference and Young Scientist School "Magnetic resonance imaging in biomedical research"

物理学;医药卫生

Fedorishin, D.^1 ; Sorokina, I.^2 ; Tolstikova, T.^2 ; Akulov, A.^1,3 ; Glazacheva, V.^1 ; Nemirovich-Danchenko, N.^1 ; Khodanovich, M.^1 ; Yarnykh, V.^1,4

Research Institute of Biology and Biophysics, Tomsk State University, Tomsk, Russia^1

Vorozhtsov Novosibirsk Institute of Organic Chemistry, Novosibirsk, Russia^2

Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia^3

Vascular Imaging Laboratory, Department of Radiology, University of Washington, Seattle

WA, United States^4

关键词: Astrogliosis;    Control groups;    Control level;    Corpus callosum;    Glial fibrillary acidic proteins;    Insulin-like growth factor 1;    Multiple sclerosis;    T2-weighted images;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/886/1/012005/pdf
DOI  :  10.1088/1742-6596/886/1/012005

学科分类：卫生学

来源: IOP

【 摘 要 】

The study aims to evaluate the effect of insulin-like growth factor 1 (IGF-1) on the demyelination and astrogliosis using the cuprizone murine model. Demyelination was induced in 14 adult male mice by 0.3% cuprizone in drinking water. Five animals from the cuprizone-treated group received subcutaneous injections of IGF-1. Seven animals were used as a control group. The extent of demyelination was evaluated as a decrease in the size of the corpus callosum on T2-weighted images that were received using an 11.7T animal MRI scanner. Brain sections were immunohistochemically stained for glial fibrillary acidic protein (GFAP), a marker of astrocytes. It was revealed that the cuprizone caused extensive demyelination and astroglyosis. IGF-1 treatment restored the size of the corpus callosum and the number of astrocytes in the corpus callosum and the anterior commissure to the control level.