The increase in the number of astrocytes in the total cerebral ischemia model in rats

3rd International Conference and Young Scientist School "Magnetic resonance imaging in biomedical research"

物理学;医药卫生

Kudabayeva, M.^1 ; Kisel, A.^1 ; Chernysheva, G.^2 ; Smol'Yakova, V.^2 ; Plotnikov, M.^2 ; Khodanovich, M.^1

Research Institute of Biology and Biophysics, Tomsk State University, Tomsk, Russia^1

E D Goldberg Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia^2

关键词: Astrogliosis;    Brain ischemia;    Brain strokes;    Cerebral ischemia;    Dentate gyrus;    Functional changes;    Granular layer;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/886/1/012009/pdf
DOI  :  10.1088/1742-6596/886/1/012009

学科分类：卫生学

来源: IOP

【 摘 要 】

Astrocytes are the most abundant cell class in the CNS. Astrocytic therapies have a huge potential for neuronal repair after stroke. The majority of brain stroke studies address the damage to neurons. Modern studies turn to the usage of morphological and functional changes in astroglial cells after stroke in regenerative medicine. Our study is focused on the changes in the number of astrocytes in the hippocampus (where new glia cells divide) after brain ischemia. Ischemia was modeled by occlusion of tr. brachiocephalicus, a. subclavia sin., a. carotis communis sin. Astrocytes were determined using immunohistochemical labeling with anti GFAP antibody. We found out that the number of astrocytes increased on the 10thand 30thdays after stroke in the CA1, CA2 fields, the granular layer of dentate gyrus (GrDG) and hilus. The morphology of astrocytes became reactive in these regions. Therefore, our results revealed long-term reactive astrogliosis in the hippocampus region after total ischemia in rats.