MRI study of the cuprizone-induced mouse model of multiple sclerosis: demyelination is not found after co-treatment with polyprenols (long-chain isoprenoid alcohols)

2nd International Conference and Young Scientist School "Magnetic resonance imaging in biomedical research"

Khodanovich, M.^1 ; Glazacheva, V.^1 ; Pan, E.^1 ; Akulov, A.^1,2 ; Krutenkova, E.^1 ; Trusov, V.^3 ; Yarnykh, V.^1,4

Neurobiology Lab, Research Institute of Biology and Biophysics, Tomsk State University, Tomsk, Russia^1

Solagran Limited, Biotechnology Company, South Melbourne, Australia^2

Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia^3

Vascular Imaging Lab, Department of Radiology, University of Washington, Seattle

WA, United States^4

关键词: Co treatments;    Control groups;    Corpus callosum;    Effective therapy;    Intraperitoneal injections;    Multiple sclerosis;    Neurological disorders;    Pathogenic mechanisms;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/677/1/012007/pdf
DOI  :  10.1088/1742-6596/677/1/012007

来源: IOP

【 摘 要 】

Multiple sclerosis is a neurological disorder with poorly understood pathogenic mechanisms and a lack of effective therapies. Therefore, the search for new MS treatments remains very important. This study was performed on a commonly used cuprizone animal model of multiple sclerosis. It evaluated the effect of a plant-derived substance called Ropren® (containing approximately 95% polyprenols or long-chain isoprenoid alcohols) on cuprizone- induced demyelination. The study was performed on 27 eight-week old male CD-1 mice. To induce demyelination mice were fed 0.5% cuprizone in the standard diet for 10 weeks. Ropren® was administered in one daily intraperitoneal injection (12mg/kg), beginning on the 6thweek of the experiment. On the 11thweek, the corpus callosum in the brain was evaluated in all animals using magnetic resonance imaging with an 11.7 T animal scanner using T2- weighted sequence. Cuprizone treatment successfully induced the model of demyelination with a significant decrease in the size of the corpus callosum compared with the control group (p