Drug resistance profile and biofilm forming potential of Pseudomonas aeruginosa isolated from contact lenses in Karachi-Pakistan

BMC Ophthalmology

Research Article

Tarrunum R Siddiqui¹  Asma Bashir²  Shahana U Kazmi³  Syed H Abidi⁴  Sikandar K Sherwani⁵ 

[1] Akhtar Eye Hospital, and Pakistan Medical Research Council, Karachi, Pakistan;Department of Biosciences, Shaheed Zulfikar Ali Bhutto Institute of Science and Technology (SZABIST), Karachi, Pakistan;Immunology and Infectious Diseases Research Lab, Department of Microbiology, University of Karachi, Karachi, Pakistan;Immunology and Infectious Diseases Research Lab, Department of Microbiology, University of Karachi, Karachi, Pakistan;Department of Biological and Biomedical Sciences, Aga Khan University, Stadium Road, 74800, Karachi, Pakistan;Immunology and Infectious Diseases Research Lab, Department of Microbiology, University of Karachi, Karachi, Pakistan;Department of Microbiology, Federal Urdu University of Arts, Science and Technology, Karachi, Pakistan;

关键词: Antibiogram;    Biofilm;    Pseudomonas aeruginosa;    Contact lens;   

DOI  :  10.1186/1471-2415-13-57

 received in 2013-01-11, accepted in 2013-10-09,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe contaminated contact lens provides Pseudomonas aeruginosa an ideal site for attachment and biofilm production. Continuous contact of the eye to the biofilm-infested lens can lead to serious ocular diseases, such as keratitis (corneal ulcers). The biofilms also prevent effective penetration of the antibiotics, which increase the chances of antibiotic resistance.MethodsFor this study, 22 Pseudomonas aeruginosa isolates were obtained from 36 contact lenses and 14 contact lens protective fluid samples. These isolates were tested against eight commonly used antibiotics using Kirby-Bauer disk diffusion method. The biofilm forming potential of these isolates was also evaluated using various qualitative and quantitative techniques. Finally, a relationship between biofilm formation and antibiotic resistance was also examined.ResultsThe isolates of Pseudomonas aeruginosa tested were found resistant to most of the antibiotics tested. Qualitative and quantitative biofilm analysis revealed that most of the isolates exhibited strong biofilm production. The biofilm production was significantly higher in isolates that were multi-drug resistant (p < 0.0001).ConclusionOur study indicates that multi-drug resistant, biofilm forming Pseudomonas aeruginosa isolates are mainly involved in contact lens associated infections. This appears to be the first report from Pakistan, which analyzes both antibiotic resistance profile and biofilm forming potential of Pseudomonas aeruginosa isolates from contact lens of the patients with contact lens associated infections.

【 授权许可】

CC BY   
© Abidi et al.; licensee BioMed Central Ltd. 2013