Anti-angiogenic potential of an ethanol extract of Annona atemoya seeds in vitro and in vivo

BMC Complementary and Alternative Medicine

Research Article

Jin Tae Hong¹  Ok-Sun Bang²  Jun Lee²  Jong-Shik Park²  Jin-Mu Yi³  No Soo Kim⁴ 

[1] College of Pharmacy, Chungbuk National University, Cheongju, Republic of Korea;KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, 305-811, Yuseong-gu, Daejeon, Republic of Korea;KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, 305-811, Yuseong-gu, Daejeon, Republic of Korea;College of Pharmacy, Chungbuk National University, Cheongju, Republic of Korea;KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, 305-811, Yuseong-gu, Daejeon, Republic of Korea;Department of Korean Medicine Life Science and Technology, Korea University of Science and Technology, Daejeon, Republic of Korea;

关键词: Annona atemoya;    Angiogenesis;    Anticancer;    HIF;    VEGF;   

DOI  :  10.1186/1472-6882-14-353

 received in 2014-06-17, accepted in 2014-09-19,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundAngiogenesis, which is initiated by certain tumor micro-environmental conditions and diverse protein factors, plays a pivotal role during tumor development and metastasis. Therefore, many efforts have been made to develop effective anti-angiogenic agents as anticancer therapeutics. In the current study, we investigated the anti-angiogenic potential of an ethanol extract of Annona atemoya seeds (EEAA) in vitro and in vivo.MethodsThe anti-angiogenic potential of EEAA was evaluated using various in vitro/in vivo models, including cell proliferation, migration, and tube formation by human umbilical vascular endothelial cells (HUVECs); a Matrigel plug assay; and tumor-induced angiogenesis. The expression of hypoxia-inducible factors (HIFs) and vascular endothelial growth factor (VEGF) was investigated using reverse transcription-polymerase chain reaction, immunoassays, and western blotting.ResultsEEAA was able to significantly inhibit the angiogenic properties of HUVECs in vitro as well as angiogenic factor-induced blood vessel formation in vivo. EEAA down-regulated the expression of VEGF and HIF-1alpha/2alpha at the mRNA and protein levels, respectively, in cancer cells under hypoxic conditions.ConclusionsEEAA shows a strong anti-angiogenic potential in both in vitro and in vivo systems, and we suggest that EEAA may be a valuable herbal source for anticancer drug development.

【 授权许可】

Unknown   
© Yi et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.