Maspin impairs the function of endothelial cells: an implying pathway of preeclampsia

BMC Pregnancy and Childbirth

Research Article

Fuyuan Qiao¹  Haiyi Liu¹  Ying Zhang¹  Yuanyuan Wu¹  Wanjiang Zeng¹  Dongrui Deng¹  Xinwei Shi¹  Ling Feng¹  Hao Liu² 

[1] Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China;Department of Urology, Wuhan Third Hospital, Guanggu on campus, Wuhan, Hubei, China;

关键词: HUVECs;    Maspin;    Hypoxia;    Angiogenesis;    Preeclampsia;   

DOI  :  10.1186/s12884-017-1525-z

 received in 2016-11-07, accepted in 2017-09-20,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroudWidespread endothelial injury contributes to the occurrence of preeclampsia. Maspin, first identified as a tumor suppressor, plays a critical role in cell invasion and angiogenesis. Our previous studies found that the expression of maspin was increased in preeclampsic placenta. In this research, we studied the function of human umbilical vein endothelial cells (HUVECs) to explore the role and possible mechanism of maspin gene in the pathogenesis of preeclampsia.MethodsHUVECs were treated with different concentration of recombinant human maspin protein (r-maspin) during normoxia and hypoxia, we detected the proliferation, apoptosis, migration and tube formation of HUVECs. We also assessed nitride oxide (NO) synthesis and the expression of matrix metalloproteinase 2 (MMP2) to further explore the underlying molecular mechanism.ResultsThere was only slight maspin expression at mRNA level in HUVECs. Treated HUVECs with r-maspin, the proliferation of HUVECs was significantly promoted both under normoxia and hypoxia. The tubes formed by HUVECs were significantly inhibited and NO synthesis was significantly reduced by r-maspin. Meantime, r-maspin also inhibited MMP2 expression and activity in HUVECs. However, there was no significant change in the migration and apoptosis of HUVECs.ConclusionsMaspin may be an important participant for mediating endothelial function and ultimately leads to the occurence of preeclamsia.

【 授权许可】

CC BY   
© The Author(s). 2017