ZFAT is essential for endothelial cell assembly and the branch point formation of capillary-like structures in an angiogenesis model

Cellular & Molecular Biology Letters

Toshiyuki Tsunoda³  Keiko Doi³  Akinori Iwasaki⁴  Masahide Kuroki¹  Yasuhiro Yoshida⁵  Takahiro Fujimoto³  Yasuo Takashima³  Senji Shirasawa³  Takehiko Sasazuki² 

[1] Center for Advanced Molecular Medicine, Fukuoka University, Fukuoka, Japan$$;Kyushu University, Fukuoka, Japan$$;Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan$$Center for Advanced Molecular Medicine, Fukuoka University, Fukuoka, Japan$$;Department of Thoracic, Endocrine and Pediatric Surgery, Faculty of Medicine, Fukuoka University, Fukuoka, Japan$$;Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan$$Department of Thoracic, Endocrine and Pediatric Surgery, Faculty of Medicine, Fukuoka University, Fukuoka, Japan$$

关键词: ZFAT;    HUVECs;    Endothelial cell assembly;    Branch point formation;    Vascular remodeling;    Angiogenesis;   

DOI  :  10.2478/s11658-010-0028-y

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

ZFAT, originally identified as a susceptibility gene for autoimmune thyroid disease, encodes a transcriptional regulator with one AT-hook and 18 C2H2-type zinc-finger domains. It is highly conserved among species. Here, we demonstrate that ZFAT is clearly expressed in human umbilical vein endothelial cells (HUVECs). Furthermore, we show that endothelial cell assembly and the branch point formation of capillary-like structures in HUVECs is impaired by the reduction of ZFAT expression through the use of ZFAT-miRNAs, whereas differences in cell proliferation or apoptotic features were not observed after the reduction in ZFAT expression. These results suggest that ZFAT may have critical roles in the capillary-like network formation that is involved in vascular remodeling. Elucidating the ZFAT-mediated transcriptional network will lead to a better understanding of the molecular mechanisms of angiogenesis.

【 授权许可】

Unknown