Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts

BMC Complementary and Alternative Medicine

Research Article

Elizabeth Barbosa¹  Manuel Jimenez-Estrada²  Karla Stephanie Martinez-Elizalde³  Angel Duran-Diaz³  Margarita Canales-Martinez³  Luis Barbo Hernandez⁴  Cesar Mateo Flores⁴  Marco Aurelio Rodriguez-Monroy⁵  Oscar J Nieto-Yañez⁵  Rocio Rosas-Lopez⁶ 

[1] Escuela Superior de Medicina IPN, Distrito Federal, Mexico;Instituto de Quimica, UNAM, Distrito Federal, Mexico;Laboratorio de Farmacognosia, Unidad de Biotecnologia y Prototipos (UBIPRO) Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de Mexico, Av. De los Barrios No.1, Los Reyes Iztacala, Edo. Mex, C.P. 54090, Tlalnepantla, Mexico;Laboratorio de Fisiología Vegetal, UBIPRO Facultad de Estudios Superiores-Iztacala UNAM, Edo. Mex, Tlalnepantla, Mexico;Laboratorio de Inmunobiología, Carrera de Medicina, Facultad de Estudios Superiores-Iztacala UNAM, Edo. Mex, Tlalnepantla, Mexico;Universidad de la Cañada, Teotitlan de Flores Magon, Oaxaca, Mexico;

关键词: Cyrtocarpa pocera;    Anacardiaceae;    Medicinal fruit;    Antibacterial activity;   

DOI  :  10.1186/s12906-015-0602-y

 received in 2014-09-23, accepted in 2015-03-09,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe fruit of Cyrtocarpa procera is used to treat stomach diseases by people living in San Rafael, Coxcatlan, Puebla. This work investigated the antibacterial, antioxidant, cytotoxic and anti-inflammatory activities of the fruit produced by this species.MethodsMethanol extract was obtained by maceration. After obtaining the methanol extract (MeOH1), methanol subextract (MeOH2) and hexane (H) were obtained. The antibacterial activities of MeOH1, MeOH2 and H were evaluated through disc-diffusion. The quenching of free radicals was evaluated by decolorizing a methanolic DPPH solution. The cytotoxic activity of MeOH2 was evaluated by in vitro assay system of growth inhibition of human cervical carcinoma cell line (CasKi). The IL-1β and TNF-α were determined through ELISA in the supernatants of the macrophage cell line (RAW 264.7). The MeOH2 subextract was separated by column chromatography, seventy-three fractions were collected.ResultsThe Gram-positive and -negative bacteria examined were sensitive to MeOH1 and MeOH2; the MeOH2 was bactericidal toward Staphyloccocus aureus (MIC = 4 mg/mL) and Vibrio cholera (MIC = 4 mg/mL). The MeOH2 inhibited the DPPH radical (SC50 = 69.7 μg/mL), but a cytotoxicity assay revealed that the extract is not toxic according to the National Cancer Institute (LD50 = 22.03 μg/mL). The production of proinflammatory cytokines (IL- 1β and TNF- α) by LPS- stimulated macrophages was reduced after the treatments. The methanol extract contained various organic acids, such as citric acid, palmitic acid and α- linoleic acid.ConclusionsThe fruits of Cyrtocarpa procera are employed to treat ailments such as diarrhea, in this study were demonstrated some biological activities involved in a bacterial infection. This is the first research about of the medicinal properties of C. procera fruit.

【 授权许可】

Unknown   
© Martinez-Elizalde et al.; licensee BioMed Central. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.