Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts

BMC Complementary and Alternative Medicine

Margarita Canales-Martinez⁴  Marco Aurelio Rodriguez-Monroy¹  Elizabeth Barbosa⁶  Oscar J Nieto-Yañez¹  Angel Duran-Diaz⁴  Rocio Rosas-Lopez²  Luis Barbo Hernandez⁵  Cesar Mateo Flores⁵  Manuel Jimenez-Estrada³  Karla Stephanie Martinez-Elizalde⁴ 

[1] Laboratorio de Inmunobiología, Carrera de Medicina, Facultad de Estudios Superiores-Iztacala UNAM, Edo. Mex, Tlalnepantla, Mexico;Universidad de la Cañada, Teotitlan de Flores Magon, Oaxaca, Mexico;Instituto de Quimica, UNAM, Distrito Federal, Mexico;Laboratorio de Farmacognosia, Unidad de Biotecnologia y Prototipos (UBIPRO) Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de Mexico, Av. De los Barrios No.1, Los Reyes Iztacala, Edo. Mex, Tlalnepantla C.P. 54090, Mexico;Laboratorio de Fisiología Vegetal, UBIPRO Facultad de Estudios Superiores-Iztacala UNAM, Edo. Mex, Tlalnepantla, Mexico;Escuela Superior de Medicina IPN, Distrito Federal, Mexico

关键词: Antibacterial activity;    Medicinal fruit;    Anacardiaceae;    Cyrtocarpa pocera;   

Others  :  1141256
DOI  :  10.1186/s12906-015-0602-y

 received in 2014-09-23, accepted in 2015-03-09,  发布年份 2015

【 摘 要 】

Background

The fruit of Cyrtocarpa procera is used to treat stomach diseases by people living in San Rafael, Coxcatlan, Puebla. This work investigated the antibacterial, antioxidant, cytotoxic and anti-inflammatory activities of the fruit produced by this species.

Methods

Methanol extract was obtained by maceration. After obtaining the methanol extract (MeOH1), methanol subextract (MeOH2) and hexane (H) were obtained. The antibacterial activities of MeOH1, MeOH2 and H were evaluated through disc-diffusion. The quenching of free radicals was evaluated by decolorizing a methanolic DPPH solution. The cytotoxic activity of MeOH2 was evaluated by in vitro assay system of growth inhibition of human cervical carcinoma cell line (CasKi). The IL-1β and TNF-α were determined through ELISA in the supernatants of the macrophage cell line (RAW 264.7). The MeOH2 subextract was separated by column chromatography, seventy-three fractions were collected.

Results

The Gram-positive and -negative bacteria examined were sensitive to MeOH1 and MeOH2; the MeOH2 was bactericidal toward Staphyloccocus aureus (MIC = 4 mg/mL) and Vibrio cholera (MIC = 4 mg/mL). The MeOH2 inhibited the DPPH radical (SC₅₀ = 69.7 μg/mL), but a cytotoxicity assay revealed that the extract is not toxic according to the National Cancer Institute (LD₅₀ = 22.03 μg/mL). The production of proinflammatory cytokines (IL- 1β and TNF- α) by LPS- stimulated macrophages was reduced after the treatments. The methanol extract contained various organic acids, such as citric acid, palmitic acid and α- linoleic acid.

Conclusions

The fruits of Cyrtocarpa procera are employed to treat ailments such as diarrhea, in this study were demonstrated some biological activities involved in a bacterial infection. This is the first research about of the medicinal properties of C. procera fruit.

【 授权许可】

   
2015 Martinez-Elizalde et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

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