Expression of micro-RNAs and genes related to angiogenesis in ccRCC and associations with tumor characteristics

BMC Urology

Research Article

Katia Ramos Moreira Leite¹  Daniel Kanda Abe¹  Ruan César Aparecido Pimenta¹  Nayara Izabel Viana¹  Renato Fidelis Ivanovic¹  Sabrina Thalita Reis¹  Rita de Cássia Oliveira¹  Vanessa Ribeiro Guimarães¹  Denis Reis Morais¹  Miguel Srougi¹  José Pontes Junior²  William Nahas³  Adriano João Nesrallah³ 

[1] Laboratory of Medical Investigation (LIM55), Urology Department, University of Sao Paulo Medical School, Av. Dr. Arnaldo 455, 2° floor, room 2145, 01246-903, Sao Paulo, Brazil;Laboratory of Medical Investigation (LIM55), Urology Department, University of Sao Paulo Medical School, Av. Dr. Arnaldo 455, 2° floor, room 2145, 01246-903, Sao Paulo, Brazil;Uro-Oncology Group, Urology Department, University of Sao Paulo Medical School and Institute of Cancer Estate of Sao Paulo (ICESP), Sao Paulo, Brazil;Uro-Oncology Group, Urology Department, University of Sao Paulo Medical School and Institute of Cancer Estate of Sao Paulo (ICESP), Sao Paulo, Brazil;

关键词: Angiogenesis;    Genes;    microRNAs;    Renal cell carcinoma clear cell type;   

DOI  :  10.1186/s12894-017-0306-3

 received in 2017-03-31, accepted in 2017-11-22,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundClear cell renal cell carcinoma (ccRCC) is the third most common urological cancer in adults. Our aim is to evaluate genes and miRNAs expression profiles involved with angiogenesis and tumor characteristics in ccRCC.MethodsThe expression levels of miRNAs miR-99a, 99b, 100; 199a; 106a; 106b; 29a; 29b; 29c; 126; 200a, 200b and their respective target genes: mTOR, HIF1-α, VHL, PDGF, VEGF, VEGFR1 and VEGFR2 were analyzed using qRT-PCR in tumor tissue samples from 56 patients with ccRCC. Five samples of benign renal tissue were utilized as control. The expression levels of miRNAs and genes were related to tumor size, Fuhrman nuclear grade and microvascular invasion.ResultsmiR99a was overexpressed in most samples and its target gene mTOR was underexpressed, this also occurs for miRNAs 106a, 106b, and their target gene VHL. An increase in miR-200b was correlated with high-risk tumors (p = 0.01) while miR-126 overexpression was associated with Fuhrman’s low grade (p = 0.03).ConclusionsOur results show that in ccRCC there are changes in miRNAs expression affecting gene expression that could be important in determining the aggressiveness of this lethal neoplasia.

【 授权许可】

CC BY   
© The Author(s). 2017