Circulating granulysin levels in healthcare workers and latent tuberculosis infection estimated using interferon-gamma release assays

BMC Infectious Diseases

Research Article

Shinsaku Sakurada¹  Le Thi Hong²  Do Bang Tam²  Pham Thu Anh³  Minako Hijikata⁴  Ikumi Matsushita⁴  Naoto Keicho⁵  Vu Cao Cuong⁶  Luu Thi Lien⁶  Pham Huu Thuong⁷  Nguyen Thi Le Hang⁸  Phan Thi Minh Ngoc⁸ 

[1] Bureau of International Medical Cooperation, National Center for Global Health and Medicine, Tokyo, Japan;Department of Biochemistry, Hematology and Blood Transfusion, Hanoi Lung Hospital, Hanoi, Vietnam;Department of National Tuberculosis Program, Hanoi Lung Hospital, Hanoi, Vietnam;Department of Pathophysiology and Host Defense, The Research Institute of Tuberculosis JATA, Tokyo, Japan;Department of Pathophysiology and Host Defense, The Research Institute of Tuberculosis JATA, Tokyo, Japan;National Center for Global Health and Medicine, Tokyo, Japan;Hanoi Department of Health, Hanoi, Vietnam;Hanoi Lung Hospital, Hanoi, Vietnam;NCGM-BMH Medical Collaboration Center, Hanoi, Vietnam;

关键词: Granulysin;    Serum concentration;    Latent tuberculosis infection;    Gene expression;    Genotype;    Biomarker;   

DOI  :  10.1186/s12879-016-1911-6

 received in 2016-04-21, accepted in 2016-10-11,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundGranulysin (GNLY) is produced by human lymphocyte subpopulations and exhibits antimicrobial activity against Mycobacterium tuberculosis. We examined the association between GNLY levels in blood and latent tuberculosis (TB) infection.MethodsLatency of TB infection among Vietnamese healthcare workers was estimated using interferon-gamma release assays (IGRA), and serum GNLY concentrations were measured using enzyme-linked immunosorbent assays. The levels of GNLY expression in whole blood and the presence of GNLY alleles with the exon-4 polymorphism rs11127 were also determined using PCR-based methods.ResultsAmong 109 study participants, 41 (37.6 %) were IGRA positive and had significantly lower serum GNLY concentrations compared with IGRA-negative participants (adjusted mean, 95 % confidence interval; 2.03, 1.72–2.44 vs. 2.48, 2.10–2.92 ng/ml, P = 0.0127; analysis of covariance). Serum GNLY concentrations and TB antigen-stimulated interferon-gamma values were weakly inversely correlated (r = −0.20, P = 0.0333). Serum GNLY concentrations varied with GNLY genotypes even after adjustment for gender and age (adjusted P = 0.0015) and were moderately correlated with GNLY expression in blood cells (r = 0.40, P < 0.0001). In subsequent analyses, low serum GNLY concentrations were significantly associated with IGRA status (adjusted odds ratio and 95 % confidence interval, 0.55 and 0.31–0.98, respectively), although GNLY genotype and mRNA levels were not.ConclusionsDecreased GNLY, presumably at the protein level, is linked to the immunological condition of latent TB infection.

【 授权许可】

CC BY   
© The Author(s). 2016