Neuroprotective effect of the hairy root extract of Angelica gigas NAKAI on transient focal cerebral ischemia in rats through the regulation of angiogenesis

BMC Complementary and Alternative Medicine

Research Article

Hyo Won Jung¹  Tae Woo Oh²  Yong-Ki Park²  Ki-Ho Park³ 

[1] Department of Herbology, College of Korean Medicine, Dongguk University, 780-714, Gyeongju, South Korea;Department of Herbology, College of Korean Medicine, Dongguk University, 780-714, Gyeongju, South Korea;Korean Medicine R&D Center, Dongguk University, 780-714, Gyeongju, Republic of Korea;Korean Medicine R&D Center, Dongguk University, 780-714, Gyeongju, Republic of Korea;

关键词: Angelica gigas;    Korean angelica;    Focal cerebral ischemia;    Angiogenesis;    Blood-brain barrier;    Neuroprotection;   

DOI  :  10.1186/s12906-015-0589-4

 received in 2013-11-18, accepted in 2015-02-25,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundIn this study, we investigated the neuroprotective effect of the hairy root extract of Angelica gigas NAKAI (Angelica Gigantis Radix) on transient focal cerebral ischemia in rats through the regulation of angiogenesis molecules.MethodsMale Sprague-Dawley rats were induced focal cerebral ischemia by a transient middle cerebral artery occlusion (tMCAO) for 90 min, and then orally administrated with the water extract of A. gigas hairy roots (AG). After 24 h reperfusion, infarction volume and the changes of BBB permeability were measured by TTC and Evans Blue (EB) staining. The neuronal cell damage and the activation of glial cells were assessed by immunohistochemistry in the ischemic brain. The expression of angiogenesis-induced proteins such as angiopoietin-1 (Ang-1), and vascular endothelial growth factor (VEGF), inflammatory protein such as intercellular adhesion molecule-1 (CAM-1), tight junction proteins such as ZO-1, and Occludin and the phosphorylation of phosphatidylinositol 3-kinase (PI3K)/AKT were determined in the ischemic brains by Western blot, respectively.ResultsThe treatment of AG extract significantly decreased the volumes of brain infarction, and edema in MACO-induced ischemic rats. AG extract decreased the increase of BBB permeability, and neuronal death and inhibited the activation of astrocytes and microglia in ischemic brains. AG extract also significantly increased the expression of Ang-1, Tie-2, VEGF, ZO-1 and Occludin through activation of the PI3K/Akt pathway. AG extract significantly increased the expression of ICAM-1 in ischemic brains.ConclusionsOur results indicate that the hairy root of AG has a neuroprotective effect in ischemic stroke.

【 授权许可】

Unknown   
© Oh et al.; licensee BioMed Central. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.