Neuroprotective effect of the hairy root extract of Angelica gigas NAKAI on transient focal cerebral ischemia in rats through the regulation of angiogenesis

BMC Complementary and Alternative Medicine

Yong-Ki Park¹  Hyo Won Jung²  Ki-Ho Park¹  Tae Woo Oh¹ 

[1] Korean Medicine R&D Center, Dongguk University, Gyeongju 780-714, Republic of Korea;Department of Herbology, College of Korean Medicine, Dongguk University, Gyeongju 780-714, South Korea

关键词: Neuroprotection;    Blood-brain barrier;    Angiogenesis;    Focal cerebral ischemia;    Korean angelica;    Angelica gigas;   

Others  :  1172061
DOI  :  10.1186/s12906-015-0589-4

 received in 2013-11-18, accepted in 2015-02-25,  发布年份 2015

【 摘 要 】

Background

In this study, we investigated the neuroprotective effect of the hairy root extract of Angelica gigas NAKAI (Angelica Gigantis Radix) on transient focal cerebral ischemia in rats through the regulation of angiogenesis molecules.

Methods

Male Sprague-Dawley rats were induced focal cerebral ischemia by a transient middle cerebral artery occlusion (tMCAO) for 90 min, and then orally administrated with the water extract of A. gigas hairy roots (AG). After 24 h reperfusion, infarction volume and the changes of BBB permeability were measured by TTC and Evans Blue (EB) staining. The neuronal cell damage and the activation of glial cells were assessed by immunohistochemistry in the ischemic brain. The expression of angiogenesis-induced proteins such as angiopoietin-1 (Ang-1), and vascular endothelial growth factor (VEGF), inflammatory protein such as intercellular adhesion molecule-1 (CAM-1), tight junction proteins such as ZO-1, and Occludin and the phosphorylation of phosphatidylinositol 3-kinase (PI3K)/AKT were determined in the ischemic brains by Western blot, respectively.

Results

The treatment of AG extract significantly decreased the volumes of brain infarction, and edema in MACO-induced ischemic rats. AG extract decreased the increase of BBB permeability, and neuronal death and inhibited the activation of astrocytes and microglia in ischemic brains. AG extract also significantly increased the expression of Ang-1, Tie-2, VEGF, ZO-1 and Occludin through activation of the PI3K/Akt pathway. AG extract significantly increased the expression of ICAM-1 in ischemic brains.

Conclusions

Our results indicate that the hairy root of AG has a neuroprotective effect in ischemic stroke.

【 授权许可】

   
2015 Oh et al.; licensee BioMed Central.

【 图 表 】

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