Overexpression of UNC5B in bladder cancer cells inhibits proliferation and reduces the volume of transplantation tumors in nude mice

BMC Cancer

Research Article

Qingchang Li¹  Bo Zhan²  Chuize Kong²  Chiyuan Piao²  Zhe Zhang²  Yuyan Zhu² 

[1] Department of Pathology, The First Hospital of China Medical University, 155 Nanjing North Street, Heping District, 110001, Shenyang City, Liaoning Province, People’s Republic of China;Department of Urology, The First Hospital of China Medical University, 155 Nanjing North Street, Heping District, 110001, Shenyang City, Liaoning Province, People’s Republic of China;

关键词: UNC5B;    Bladder cancer;    Cell cycle;    Migration;   

DOI  :  10.1186/s12885-016-2922-9

 received in 2016-07-01, accepted in 2016-11-03,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe netrin-1 receptor UNC5B plays vital roles in angiogenesis, inflammation, embryonic development and carcinogenesis. However, the functional significance of UNC5B overexpression in bladder cancer remains unclear. In this study, we investigated the role of UNC5B in bladder cancer in vitro and in vivo.MethodsStable transfection of the human bladder cancer cell line 5637 with UNC5B (5637-U) was confirmed by real-time RT-PCR, western blot and immunofluorescence assays. UNC5B expression in 5637 and 5637-U cells and mice tumor specimens derived from these cell lines was analyzed by immunohistochemistryand western blotting. Changes in the levels of cell cycle proteins were evaluated by western blotting. Flow cytometry, CCK-8 and scratch tests were used to examine cell cycle distribution, proliferation and migration, respectively.ResultsUNC5B overexpression in 5637 cells inhibited cell multiplication and migration and induced cell cycle arrest at the G2/M phase, meanwhile exhibited changes in the expression of cell cycle-associated proteins, showing that UNC5B may inhibit metastatic behaviors in bladder cancer cells. In addition, tumors generated from 5637-U cells were smaller than tumors generated from control 5637 cells.ConclusionsOur findings suggest that UNC5B is a potential anti-neoplastic target in bladder cancer progression.

【 授权许可】

CC BY   
© The Author(s). 2016