PKC α regulates netrin-1/UNC5B-mediated survival pathway in bladder cancer

BMC Cancer

Jiao Liu¹  Chui-ze Kong¹  Da-xin Gong¹  Zhe Zhang¹  Yu-yan Zhu¹ 

[1] Department of Urology, the First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, China

关键词: Bladder cancer;    UNC5B;    Netrin-1;    PKC α;   

Others  :  859064
DOI  :  10.1186/1471-2407-14-93

 received in 2013-06-12, accepted in 2014-02-11,  发布年份 2014

【 摘 要 】

Background

Netrin-1 and its receptor UNC5B play important roles in angiogenesis, embryonic development, cancer and inflammation. However, their expression patttern and biological roles in bladder cancer have not been well characterized. The present study aims to investigating the clinical significance of PKC α, netrin-1 and UNC5B in bladder cancer as well as their association with malignant biological behavior of cancer cells.

Methods

Netrin-1 and UNC5B expression was examined in 120 bladder cancer specimens using immunohistochemistry and in 40 fresh cancer tissues by western blot. Immunofluorescence was performed in cancer cell lines. PKC α agonist PMA and PKC siRNA was employed in bladder cancer cells. CCK-8, wound healing assays and flow cytometry analysis were used to examine cell proliferation, migration and cell cycle, respectively.

Results

Netrin-1 expression was positively correlated with histological grade, T stage, metastasis and poor prognosis in bladder cancer tissues. Immunofluorescence showed elevated netrin-1 and decreased UNC5B expression in bladder cancer cells compared with normal bladder cell line. Furthermore, cell proliferation, migration and cell cycle progression were promoted with PMA treatment while inhibited by calphostin C. In addition, PMA treatment could induce while calphostin C reduce netrin-1 expression in bladder cancer cells.

Conclusions

The present study identified netrin-1/UNC5B, which could be regulated by PKC signaling, was important mediators of bladder cancer progression.

【 授权许可】

   
2014 Liu et al.; licensee BioMed Central Ltd.

【 图 表 】

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