Expression of netrin-1 receptors in retina of oxygen-induced retinopathy in mice

BMC Ophthalmology

Research Article

Lei Shang¹  Dan Liu²  Xiao-feng Tian²  Si-Qi Xiong²  Jing Yang²  Xiao-Bo Xia² 

[1] Department of Anatomy and Neurobiology, School of Basic Medical Sciences, Central South University, 410013, Changsha, Hunan, China;Department of Ophthalmology, Xiangya Hospital, Central South University, 410008, Changsha, Hunan, China;

关键词: Netrin-1;    UNC5B;    Neogenin;    Retinal neovascularization;   

DOI  :  10.1186/1471-2415-14-102

 received in 2014-03-23, accepted in 2014-08-18,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundNetrin-1 has been reported to promote retinal neovascularization in oxygen-induced retinopathy (OIR). However, netrin-1 receptors, which may mediate netrin-1 action during retinal neovascularization, have not been characterized. In this study, we investigated netrin-1 receptor subtype expression and associated changes in the retinas of mice with OIR.MethodsC57BL/6J mice were exposed to 75±2% oxygen for 5 days and then returned to normal air to induce retinal neovascularization. Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot were used to examine the expression of netrin-1 receptor subtypes in the mouse retinas. Double staining of netrin-1 receptor subtypes and isolectin B4 was used to determine the location of the netrin-1 receptor subtypes in the retinas. Inhibition of retinal neovascularization was achieved by UNC5B shRNA plasmid intravitreal injection. Retinal neovascularization was examined by fluorescein angiography and quantification of preretinal neovascular nuclei in retinal sections.ResultsRT-PCR results showed that, except for UNC5A, netrin-1 receptor subtypes UNC5B, UNC5C, UNC5D, DCC, neogenin, and A2b were all expressed in the retinas of OIR mice 17 days after birth. Western blots showed that only UNC5B expression was significantly increased on that day, and immunofluorescence results showed that only UNC5B and neogenin were expressed in retinal vessels. Treatment of OIR mice with the UNC5B shRNA plasmid dramatically reduced neovascular tufts and neovascular outgrowth into the inner limiting membrane.ConclusionsUNC5B may promote retinal neovascularization in OIR mice.

【 授权许可】

Unknown   
© Liu et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.