Expression of the schizophrenia associated gene FEZ1 in the early developing fetal human forebrain

Frontiers in Neuroscience

Neuroscience

Hannah Ronan¹  Maznah Alhesain¹  Fiona E. N. LeBeau¹  Gavin J. Clowry² 

[1] Centre for Transformative Research in Neuroscience, Newcastle University Biosciences Institute, Newcastle upon Tyne, United Kingdom;null;

关键词: axonogenesis;    cerebral cortex;    ganglionic eminences;    neurodevelopmental diseases (NDDs);    SNARE complex;    thalamus;    transcription;   

DOI  :  10.3389/fnins.2023.1249973

 received in 2023-06-29, accepted in 2023-08-15,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionThe protein fasciculation and elongation zeta-1 (FEZ1) is involved in axon outgrowth but potentially interacts with various proteins with roles ranging from intracellular transport to transcription regulation. Gene association and other studies have identified FEZ1 as being directly, or indirectly, implicated in schizophrenia susceptibility. To explore potential roles in normal early human forebrain neurodevelopment, we mapped FEZ1 expression by region and cell type.MethodsAll tissues were provided with maternal consent and ethical approval by the Human Developmental Biology Resource. RNAseq data were obtained from previously published sources. Thin paraffin sections from 8 to 21 post-conceptional weeks (PCW) samples were used for RNAScope in situ hybridization and immunohistochemistry against FEZ1 mRNA and protein, and other marker proteins.ResultsTissue RNAseq revealed that FEZ1 is highly expressed in the human cerebral cortex between 7.5–17 PCW and single cell RNAseq at 17–18 PCW confirmed its expression in all neuroectoderm derived cells. The highest levels were found in more mature glutamatergic neurons, the lowest in GABAergic neurons and dividing progenitors. In the thalamus, single cell RNAseq similarly confirmed expression in multiple cell types. In cerebral cortex sections at 8–10 PCW, strong expression of mRNA and protein appeared confined to post-mitotic neurons, with low expression seen in progenitor zones. Protein expression was observed in some axon tracts by 16–19 PCW. However, in sub-cortical regions, FEZ1 was highly expressed in progenitor zones at early developmental stages, showing lower expression in post-mitotic cells.DiscussionFEZ1 has different expression patterns and potentially diverse functions in discrete forebrain regions during prenatal human development.

【 授权许可】

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Copyright © 2023 Alhesain, Ronan, LeBeau and Clowry.