Transcriptional heterogeneity of ventricular zone cells in the ganglionic eminences of the mouse forebrain

eLife

Apratim Mitra¹  Ryan K Dale¹  Dragan Maric²  Timothy J Petros³  Yajun Zhang³  Christopher Rhodes³  Dongjin R Lee³ 

[1] Bioinformatics and Scientific Programming Core, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States;Flow and Imaging Cytometry Core, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States;Unit on Cellular and Molecular Neurodevelopment, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States;

关键词: interneurons;    neurodevelopment;    ganglionic eminences;    ventricular zone;    radial glia;    single cell sequencing;   

DOI  :  10.7554/eLife.71864

来源: DOAJ

【 摘 要 】

The ventricular zone (VZ) of the nervous system contains radial glia cells that were originally considered relatively homogenous in their gene expression, but a detailed characterization of transcriptional diversity in these VZ cells has not been reported. Here, we performed single-cell RNA sequencing to characterize transcriptional heterogeneity of neural progenitors within the VZ and subventricular zone (SVZ) of the ganglionic eminences (GEs), the source of all forebrain GABAergic neurons. By using a transgenic mouse line to enrich for VZ cells, we characterize significant transcriptional heterogeneity, both between GEs and within spatial subdomains of specific GEs. Additionally, we observe differential gene expression between E12.5 and E14.5 VZ cells, which could provide insights into temporal changes in cell fate. Together, our results reveal a previously unknown spatial and temporal genetic diversity of VZ cells in the ventral forebrain that will aid our understanding of initial fate decisions in the forebrain.

【 授权许可】

Unknown