Tangential Migration in Neocortical Development

Developmental Biology

David Jiménez¹  Laura M. López-Mascaraque¹  Juan A. De Carlos¹  Facundo Valverde¹ 

[1] Instituto Cajal (C.S.I.C.), Avenida del Doctor Arce 37, 28002, Madrid, Spain

关键词: development;    embryo;    ganglionic eminences;    cerebral cortex;    tangential migrations;    rat;   

DOI  :  10.1006/dbio.2002.0586

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Duringcorticaldevelopment,differentcellpopulationsariseinthebasaltelencephalonandsubsequentlymigratetangentiallytotheneocortex.However,itisnotclearwhetherthesecorticalcellsaregeneratedinthelateralganglioniceminence(LGE),themedialganglioniceminence(MGE),orboth.Inthisstudy,wehavegeneratedathree-dimensionalreconstructiontostudythemorphologicalformationofthetwoganglioniceminencesandtheinterganglionicsulcus.Asaresult,wehavedemonstratedtheimportanceofthedevelopmentofthesestructuresforthistangentialmigrationtotheneocortex.WehavealsousedthetracersDiIandBDAinmultipleexperimentalparadigms(wholeembryoculture,inuteroinjections,andbrainslicecultures)toanalyzetheroutesofcellmigrationandtodemonstratetherolesofbotheminencesinthedevelopmentofthecerebralcortex.Theseresultsarefurtherstrengthened,confirmingtheimportanceoftheMGEinthismigrationanddemonstratingtheearlygenerationoftangentialmigratorycellsintheLGEearlyindevelopment.Finally,weshowthatthecalcium-bindingproteinCalretininisexpressedinsomeofthesetangentiallymigratingcells.Moreover,wedescribethespatiotemporalsequenceofGABA,Calbindin,andCalretininexpression,showingthatthesethreemarkersareexpressedinthecorticalneuroepitheliumoverseveralembryonicdays,suggestingthatthecellsmigratingtangentiallyformaheterogeneouspopulation.

【 授权许可】

Unknown