期刊论文详细信息
Cellular & Molecular Biology Letters
CRISPR applications in cancer diagnosis and treatment
Review Letter
Xia Wu1  Xinbo Huang1  Bo Yu1  Menghui Chen1  Mingxia Wang2 
[1] Department of Dermatology, Skin Research Institute of Peking University Shenzhen Hospital, Peking University Shenzhen Hospital, 518036, Shenzhen, China;Department of Dermatology, Skin Research Institute of Peking University Shenzhen Hospital, Peking University Shenzhen Hospital, 518036, Shenzhen, China;Shenzhen Key Laboratory of Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, 518000, Shenzhen, China;
关键词: Cas9;    Cas12;    Cas13;    Cancer;    Gene therapy;    Diagnostic tools;    Clinical trials;   
DOI  :  10.1186/s11658-023-00483-4
 received in 2023-05-11, accepted in 2023-08-17,  发布年份 2023
来源: Springer
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【 摘 要 】

Cancer remains a significant global health challenge, necessitating the exploration of novel and more precise therapeutic options beyond conventional treatments. In this regard, clustered regularly interspaced short palindromic repeats (CRISPR) systems have emerged as highly promising tools for clinical gene editing applications. The CRISPR family encompasses diverse CRISPR-associated (Cas) proteins that possess the ability to recognize specific target sequences. The initial CRISPR system consisted of the Cas9 protein and a single-guide RNA, which guide Cas9 to the desired target sequence, facilitating precise double-stranded cleavage. In addition to the traditional cis-cleavage activity, the more recently discovered Cas12 and Cas13 proteins exhibit trans-cleavage activity, which expands their potential applications in cancer diagnosis. In this review, we provide an overview of the functional characteristics of Cas9, Cas12, and Cas13. Furthermore, we highlight the latest advancements and applications of these CRISPR systems in cancer gene therapy and molecular diagnosis. We also emphasize the importance of understanding the strengths and limitations of each CRISPR system to maximize their clinical utility. By providing a comprehensive overview of the current state of CRISPR technology in cancer research, we aim to inspire further exploration and innovation in this rapidly evolving field.Graphical abstract

【 授权许可】

CC BY   
© University of Wroclav 2023

【 预 览 】
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【 图 表 】

Fig. 3

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Fig. 2

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【 参考文献 】
  • [1]
  • [2]
  • [3]
  • [4]
  • [5]
  • [6]
  • [7]
  • [8]
  • [9]
  • [10]
  • [11]
  • [12]
  • [13]
  • [14]
  • [15]
  • [16]
  • [17]
  • [18]
  • [19]
  • [20]
  • [21]
  • [22]
  • [23]
  • [24]
  • [25]
  • [26]
  • [27]
  • [28]
  • [29]
  • [30]
  • [31]
  • [32]
  • [33]
  • [34]
  • [35]
  • [36]
  • [37]
  • [38]
  • [39]
  • [40]
  • [41]
  • [42]
  • [43]
  • [44]
  • [45]
  • [46]
  • [47]
  • [48]
  • [49]
  • [50]
  • [51]
  • [52]
  • [53]
  • [54]
  • [55]
  • [56]
  • [57]
  • [58]
  • [59]
  • [60]
  • [61]
  • [62]
  • [63]
  • [64]
  • [65]
  • [66]
  • [67]
  • [68]
  • [69]
  • [70]
  • [71]
  • [72]
  • [73]
  • [74]
  • [75]
  • [76]
  • [77]
  • [78]
  • [79]
  • [80]
  • [81]
  • [82]
  • [83]
  • [84]
  • [85]
  • [86]
  • [87]
  • [88]
  • [89]
  • [90]
  • [91]
  • [92]
  • [93]
  • [94]
  • [95]
  • [96]
  • [97]
  • [98]
  • [99]
  • [100]
  • [101]
  • [102]
  • [103]
  • [104]
  • [105]
  • [106]
  • [107]
  • [108]
  • [109]
  • [110]
  • [111]
  • [112]
  • [113]
  • [114]
  • [115]
  • [116]
  • [117]
  • [118]
  • [119]
  • [120]
  • [121]
  • [122]
  • [123]
  • [124]
  • [125]
  • [126]
  • [127]
  • [128]
  • [129]
  • [130]
  • [131]
  • [132]
  • [133]
  • [134]
  • [135]
  • [136]
  • [137]
  • [138]
  • [139]
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