Advances in the application of CRISPR-Cas technology in rapid detection of pathogen nucleic acid

Frontiers in Molecular Biosciences

Molecular Biosciences

Yinbiao Qiao¹  Shusen Zheng²  Jianhui Li³  Saber Imani⁴  Xiaolei Mao⁴  Jiaye Zhong⁴  Huayan Fan⁴  Xiaoping Li⁵  Haoyu Li⁶  Yiwu Zhong⁷ 

[1] Department of Hepatobiliary and Pancreatic Surgery, Department of Liver Transplantation, Shulan (Hangzhou) Hospital, Zhejiang Shuren University School of Medicine, Hangzhou, China;NHC Key Laboratory of Combined Multi-Organ Transplantation, Hangzhou, China;Department of Hepatobiliary and Pancreatic Surgery, Department of Liver Transplantation, Shulan (Hangzhou) Hospital, Zhejiang Shuren University School of Medicine, Hangzhou, China;NHC Key Laboratory of Combined Multi-Organ Transplantation, Hangzhou, China;Jinan Microecological Biomedicine Shandong Laboratory, Jinan, China;Department of Hepatobiliary and Pancreatic Surgery, Department of Liver Transplantation, Shulan (Hangzhou) Hospital, Zhejiang Shuren University School of Medicine, Hangzhou, China;NHC Key Laboratory of Combined Multi-Organ Transplantation, Hangzhou, China;The Organ Repair and Regeneration Medicine Institute of Hangzhou, Hangzhou, China;Jinan Microecological Biomedicine Shandong Laboratory, Jinan, China;Zhejiang Chinese Medical University, Hangzhou, China;Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Shulan International Medical College, Zhejiang Shuren University, Hangzhou, China;Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Shulan International Medical College, Zhejiang Shuren University, Hangzhou, China;Faculty of Medicine, Macau University of Science and Technology, Avenida Wai Long Taipa, Macau, China;NHC Key Laboratory of Combined Multi-Organ Transplantation, Hangzhou, China;Jinan Microecological Biomedicine Shandong Laboratory, Jinan, China;Zhejiang Chinese Medical University, Hangzhou, China;

关键词: CRISPR-Cas;    Cas9;    Cas12;    Cas13;    Cas14;    pathogen nucleic acid;    rapid detection;   

DOI  :  10.3389/fmolb.2023.1260883

 received in 2023-08-06, accepted in 2023-09-06,  发布年份 2023

来源: Frontiers

【 摘 要 】

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) are widely used as gene editing tools in biology, microbiology, and other fields. CRISPR is composed of highly conserved repetitive sequences and spacer sequences in tandem. The spacer sequence has homology with foreign nucleic acids such as viruses and plasmids; Cas effector proteins have endonucleases, and become a hotspot in the field of molecular diagnosis because they recognize and cut specific DNA or RNA sequences. Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.), providing a new way for rapid detection of pathogen nucleic acid. This paper introduces the biological mechanism and classification of CRISPR-Cas technology, summarizes the existing rapid detection technology for pathogen nucleic acid based on the trans cleavage activity of Cas, describes its characteristics, functions, and application scenarios, and prospects the future application of this technology.

【 授权许可】

Unknown   
Copyright © 2023 Li, Zhong, Li, Qiao, Mao, Fan, Zhong, Imani, Zheng and Li.