期刊论文详细信息
Frontiers in Bioengineering and Biotechnology
Advancements in the synergy of isothermal amplification and CRISPR-cas technologies for pathogen detection
Bioengineering and Biotechnology
Minghui Xu1  Shuyin Luo1  Yi Yang1  Jiawei Zhou1  Xiaolei Mao1  Jiaye Zhong1  Huayan Fan1  Xiaoping Li2  Zhi Chen3 
[1] Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Shulan International Medical College, Zhejiang Shuren University, Hangzhou, China;Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Shulan International Medical College, Zhejiang Shuren University, Hangzhou, China;Faculty of Medicine, Macau University of Science and Technology, Macau, China;Shulan International Medical College, Zhejiang Shuren University, Hangzhou, China;
关键词: recombinase polymerase amplification;    recombinase-aid amplification;    CRISPR-cas;    Cas12;    Cas13;    rapid inspection technology improvement;    sherlock;    DETECTR;   
DOI  :  10.3389/fbioe.2023.1273988
 received in 2023-08-07, accepted in 2023-09-29,  发布年份 2023
来源: Frontiers
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【 摘 要 】

In the realm of pathogen detection, isothermal amplification technology has emerged as a swift, precise, and sensitive alternative to conventional PCR. This paper explores the fundamental principles of recombinase polymerase amplification (RPA) and recombinase-aid amplification (RAA) and reviews the current status of integrating the CRISPR-Cas system with RPA/RAA techniques. Furthermore, this paper explores the confluence of isothermal amplification and CRISPR-Cas technology, providing a comprehensive review and enhancements of existing combined methodologies such as SHERLOCK and DETECTR. We investigate the practical applications of RPA/RAA in conjunction with CRISPR-Cas for pathogen detection, highlighting how this integrated approach significantly advances both research and clinical implementation in the field. This paper aims to provide readers with a concise understanding of the fusion of RPA/RAA and CRISPR-Cas technology, offering insights into their clinical utility, ongoing enhancements, and the promising prospects of this integrated approach in pathogen detection.

【 授权许可】

Unknown   
Copyright © 2023 Mao, Xu, Luo, Yang, Zhong, Zhou, Fan, Li and Chen.

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