Cost-effective production of alginate oligosaccharides from Laminaria japonica roots by Pseudoalteromonas agarivorans A3

Microbial Cell Factories

Research

Xiao-Meng Sun¹  Mei-Ling Sun²  Xiao-Hui Sun³  Xin-Ru Zhang³  Xiao-Fei Wang³  Xiu-Lan Chen³  Yu-Qiang Zhang³  Yu-Zhong Zhang⁴  Fei Xu⁵  Xi-Ying Zhang⁵ 

[1] Frontiers Science Center for Deep Ocean Multispheres and Earth System & College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China;Frontiers Science Center for Deep Ocean Multispheres and Earth System & College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China;Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, 266237, Qingdao, China;State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, 266237, Qingdao, China;State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, 266237, Qingdao, China;Frontiers Science Center for Deep Ocean Multispheres and Earth System & College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China;Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, 266237, Qingdao, China;State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, 266237, Qingdao, China;Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, 266237, Qingdao, China;

关键词: Alginate oligosaccharides;    Pseudoalteromonas;    Laminaria japonica;    Fermentation;    Alginate lyase;   

DOI  :  10.1186/s12934-023-02170-7

 received in 2023-05-15, accepted in 2023-08-07,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundAlginate oligosaccharides (AOs) are the degradation products of alginate, a natural polysaccharide abundant in brown algae. AOs generated by enzymatic hydrolysis have diverse bioactivities and show broad application potentials. AOs production via enzymolysis is now generally with sodium alginate as the raw material, which is chemically extracted from brown algae. In contrast, AOs production by direct degradation of brown algae is more advantageous on account of its cost reduction and is more eco-friendly. However, there have been only a few attempts reported in AOs production from direct degradation of brown algae.ResultsIn this study, an efficient Laminaria japonica-decomposing strain Pseudoalteromonas agarivorans A3 was screened. Based on the secretome and mass spectrum analyses, strain A3 showed the potential as a cell factory for AOs production by secreting alginate lyases to directly degrade L. japonica. By using the L. japonica roots, which are normally discarded in the food industry, as the raw material for both fermentation and enzymatic hydrolysis, AOs were produced by the fermentation broth supernatant of strain A3 after optimization of the alginate lyase production and hydrolysis parameters. The generated AOs mainly ranged from dimers to tetramers, among which trimers and tetramers were predominant. The degradation efficiency of the roots reached 54.58%, the AOs production was 33.11%, and the AOs purity was 85.03%.ConclusionAn efficient, cost-effective and green process for AOs production directly from the underutilized L. japonica roots by using strain A3 was set up, which differed from the reported processes in terms of the substrate and strain used for fermentation and the AOs composition. This study provides a promising platform for scalable production of AOs, which may have application potentials in industry and agriculture.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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