Microbiota from alginate oligosaccharide-dosed mice successfully mitigated small intestinal mucositis

Microbiome

Shuai Yu¹  Yishan Gao²  Zengkuan Li²  Beining Kang²  Shunfeng Cheng³  Yanan Hao³  Wei Shen³  Bohui Xiong³  Cong Zhang³  Wei Ge³  Pengfei Zhang⁴  Xiangfang Tang⁴  Yong Zhao⁴  Hongfu Zhang⁴  Liang Chen⁴  Jing Liu⁵ 

[1] Center for Reproductive Medicine, Urology Department, Peking University Shenzhen Hospital;College of Animal Sciences and Technology, Qingdao Agricultural University;College of Life Sciences, Qingdao Agricultural University;State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences;University Research Core, Qingdao Agricultural University;

关键词: Fecal microbiota transplantation;    Alginate oligosaccharides;    Mucositis;    Busulfan;    Rescue;    Metabolome;   

DOI  :  10.1186/s40168-020-00886-x

来源: DOAJ

【 摘 要 】

Abstract Background The increasing incidence of cancer and intestinal mucositis induced by chemotherapeutics are causing worldwide concern. Many approaches such as fecal microbiota transplantation (FMT) have been used to minimize mucositis. However, it is still unknown whether FMT from a donor with beneficial gut microbiota results in more effective intestinal function in the recipient. Recently, we found that alginate oligosaccharides (AOS) benefit murine gut microbiota through increasing “beneficial” microbes to rescue busulfan induced mucositis. Results In the current investigation, FMT from AOS-dosed mice improved small intestine function over FMT from control mice through the recovery of gene expression and an increase in the levels of cell junction proteins. FMT from AOS-dosed mice showed superior benefits over FMT from control mice on recipient gut microbiotas through an increase in “beneficial” microbes such as Leuconostocaceae and recovery in blood metabolome. Furthermore, the correlation of gut microbiota and blood metabolites suggested that the “beneficial” microbe Lactobacillales helped with the recovery of blood metabolites, while the “harmful” microbe Mycoplasmatales did not. Conclusion The data confirm our hypothesis that FMT from a donor with superior microbes leads to a more profound recovery of small intestinal function. We propose that gut microbiota from naturally produced AOS-treated donor may be used to prevent small intestinal mucositis induced by chemotherapeutics or other factors in recipients. Video Abstract

【 授权许可】

Unknown