Combined transcriptomic and metabolomic analysis of alginate oligosaccharides alleviating salt stress in rice seedlings

BMC Plant Biology

Research

Hang Zhou¹  You-Wei Du¹  Ling Liu¹  Ya-xing Wang¹  Hui-Min Zhao¹  Peng Deng¹  Mei-Ling Liu¹  Nai-Jie Feng²  Dian-Feng Zheng² 

[1] College of Coastal Agriculture Sciences, Guangdong Ocean University, 524088, Zhanjiang, China;South China Center of National Saline-tolerant Rice Technology Innovation Center, 524088, Zhanjiang, China;College of Coastal Agriculture Sciences, Guangdong Ocean University, 524088, Zhanjiang, China;South China Center of National Saline-tolerant Rice Technology Innovation Center, 524088, Zhanjiang, China;Shenzhen Research Institute of Guangdong Ocean University, 518108, Shenzhen, China;

关键词: Rice;    Salt stress;    Alginate oligosaccharides;    Transcriptome;    Metabolome;   

DOI  :  10.1186/s12870-023-04470-x

 received in 2023-07-06, accepted in 2023-09-17,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundSalt stress is one of the key factors limiting rice production. Alginate oligosaccharides (AOS) enhance plant stress resistance. However, the molecular mechanism underlying salt tolerance in rice induced by AOS remains unclear. FL478, which is a salt-tolerant indica recombinant inbred line and IR29, a salt-sensitive rice cultivar, were used to comprehensively analyze the effects of AOS sprayed on leaves in terms of transcriptomic and metabolite profiles of rice seedlings under salt stress.ResultsIn this experiment, exogenous application of AOS increased SOD, CAT and APX activities, as well as GSH and ASA levels to reduce the damage to leaf membrane, increased rice stem diameter, the number of root tips, aboveground and subterranean biomass, and improved rice salt tolerance. Comparative transcriptomic analyses showed that the regulation of AOS combined with salt treatment induced the differential expression of 305 and 1030 genes in FL478 and IR29. The expressed genes enriched in KEGG pathway analysis were associated with antioxidant levels, photosynthesis, cell wall synthesis, and signal transduction. The genes associated with light-trapping proteins and RLCK receptor cytoplasmic kinases, including CBA, LHCB, and Lhcp genes, were fregulated in response to salt stress. Treatment with AOS combined with salt induced the differential expression of 22 and 50 metabolites in FL478 and IR29. These metabolites were mainly related to the metabolism of amino and nucleotide sugars, tryptophan, histidine, and β -alanine. The abundance of metabolites associated with antioxidant activity, such as 6-hydroxymelatonin, wedelolactone and L-histidine increased significantly. Combined transcriptomic and metabolomic analyses revealed that dehydroascorbic acid in the glutathione and ascorbic acid cycles plays a vital role in salt tolerance mediated by AOS.ConclusionAOS activate signal transduction, regulate photosynthesis, cell wall formation, and multiple antioxidant pathways in response to salt stress. This study provides a molecular basis for the alleviation of salt stress-induced damage by AOS in rice.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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