Correlation between antioxidant and anticancer activity and phenolic profile of new Apulian table grape genotypes (V. Vinifera L.)

Frontiers in Plant Science

Plant Science

Valeria Tutino¹  Isabella Gigante¹  Maria Notarnicola¹  Riccardo Flamini²  Mirko De Rosso²  Riccardo Velasco²  Antonio Domenico Marsico³  Rosa Anna Milella³  Rocco Perniola³  Marica Gasparro³  Giambattista Debiase³  Lucia Rosaria Forleo³ 

[1] Laboratory of Nutritional Biochemistry, National Institute of Gastroenterology Saverio de Bellis, IRCCS Research Hospital, Castellana Grotte, Italy;Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics, Conegliano, Treviso, Italy;Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics, Turi, Bari, Italy;

关键词: breeding;    phenolic compounds;    seedless grape;    antioxidant;    anticancer;    quality improvement;   

DOI  :  10.3389/fpls.2022.1064023

 received in 2022-10-07, accepted in 2022-12-16,  发布年份 2023

来源: Frontiers

【 摘 要 】

Grapes represent a significant source of phenolic compounds known for their health-promoting properties, such as antioxidant capacity on normal cells and prooxidant activity on tumor cells. The genotype highly affects the polyphenolic composition in grapes and, consequently, the nutritional quality of berries. This work aimed to characterize the phenolic composition, the antioxidant, and anticancer activity of grape skin extracts (GSEs) of nine new table grape genotypes selected from a breeding program to obtain new cultivars of seedless table grapes, well adapted to the climatic change and with higher nutraceutical properties. The grape polyphenolic profile was characterized by Ultra-High-Performance Liquid Chromatography/Quadrupole-Time of Flight mass spectrometry analysis. GSE antioxidant activity was determined by the ABTS, DPPH, and ORAC assays; GSE cell growth inhibition test was carried out in the Caco2 human cancer cell line. The nine GSEs showed different flavonoid and non-flavonoid profiles, and all possessed antioxidant activity, with the ‘Aika N.’, ‘Turese N.’, and ‘Egnatia N.’ the most active. As anticancer activity against the tested cancer cell line, ‘Daunia N.’ and ‘Apenestae N.’ showed the EC50 after 24 h of 35.60 µg/mL and 150.91 µg/mL, respectively. The relationship between polyphenolic profile and the antioxidant and anticancer activity of GSE was also investigated. Interestingly, among the different classes of polyphenolics, flavan-3-ols e proanthocyanidins showed the highest positive correlation with the anticancer activity of extracts. These findings can be helpful for the preparation of new extracts for the pharmaceutical and nutraceutical industry and geneticists working in vine breeding programs.

【 授权许可】

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Copyright © 2023 Milella, De Rosso, Gasparro, Gigante, Debiase, Forleo, Marsico, Perniola, Tutino, Notarnicola, Velasco and Flamini