Genomic characteristics of Mycobacterium tuberculosis isolates of cutaneous tuberculosis

Frontiers in Microbiology

Microbiology

Ji-Ya Sun¹  Tao Luo²  You-Ming Mei³  Jing-Shu Xiong³  Wen-Yue Zhang³  Hong-Sheng Wang³  Hai-Qin Jiang³  Ying Shi³  Chun Pan³  Le Wang³  Si-Yu Long³  Yan-Qing Chen³ 

[1] Center for Systems Medicine, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China;Suzhou Institute of Systems Medicine, Suzhou, China;Department of Pathogen Biology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China;Hospital of Skin Disease, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China;Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Nanjing, China;

关键词: mycobacteria infection;    Mycobacterium tuberculosis;    whole genome sequence;    cutaneous tuberculosis;    comparative genomics;   

DOI  :  10.3389/fmicb.2023.1165916

 received in 2023-02-14, accepted in 2023-04-19,  发布年份 2023

来源: Frontiers

【 摘 要 】

ObjectivesCutaneous tuberculosis with various manifestations can be divided into several clinical types according to the host's immune status and infective route. However, the etiological factors of this disease remain unclear. The objective of this study is to investigate the pathogens associated with the occurrence and different types of cutaneous tuberculosis.Methods58 Mycobacterium tuberculosis strains isolated from cutaneous tuberculosis over the last 20 years were sequenced and analyzed for genomic characteristics including lineage distribution, drug-resistance mutations, and mutations potentially associated with different sites of infection.ResultsThe M. tuberculosis strains from four major types of cutaneous tuberculosis and pulmonary tuberculosis shared similar genotypes and genomic composition. The strains isolated from cutaneous tuberculosis had a lower rate of drug resistance. Phylogenic analysis showed cutaneous tuberculosis and pulmonary tuberculosis isolates scattered on the three. Several SNPs in metabolism related genes exhibited a strong correlation with different infection sites.ConclusionsThe different infection sites of TB may barely be affected by large genomic changes in M. tuberculosis isolates, but the significant difference in SNPs of drug resistance gene and metabolism-related genes still deserves more attention.

【 授权许可】

Unknown   
Copyright © 2023 Mei, Zhang, Sun, Jiang, Shi, Xiong, Wang, Chen, Long, Pan, Luo and Wang.