PCR in the diagnosis of cutaneous tuberculosis

Brazilian Journal of Microbiology

Mauricio Morishi Ogusku²  Aya Sadahiro¹  Mário Hiroyuki Hirata¹  Rosário D. C. Hirata¹  Clarisse Zaitz¹  Júlia Ignez Salem² 

[1] ,Instituto Nacional de Pesquisas da Amazônia Coordenação de Pesquisas em Ciências da Saúde Manaus AM ,Brasil

关键词: Mycobacterium tuberculosis;    cutaneous tuberculosis;    Polymerase Chain Reaction (PCR);    laboratory diagnosis;    Mycobacterium tuberculosis;    tuberculose cutânea;    Reação em Cadeia da Polimerase (PCR);    diagnóstico laboratorial;   

DOI  :  10.1590/S1517-83822003000200015

来源: SciELO

【 摘 要 】

Seeking to improve the laboratory diagnosis of Cutaneous Tuberculosis, a study was carried out on the application of PCR technique in macerated, decontaminated(with 4% H2SO4 for elimination of normal microbiot), neutralized (with 4% NaOH)biopsies tissues samples stored at -20ºC. Of the 37 samples submitted for study, 16.22% were positive by microscopy for acid-fast bacilli (concentrated method) and in 43.24% the Mycobacterium tuberculosis was isolated in Löwenstein-Jensen medium. Using a M. tuberculosis complex specific primer set (gene sequence for 16S rDNA), the mycobacterial DNA was detected in 24.32% of the biopsies. The sensitivity and specificity of PCR were 43.7% and 90.4%, respectively. Due to low sensitivity and discrepant results between bacteriological techniques and PCR methodology, the samples were repeated in a new PCR with primers for the IS6110 target. The sensitivity and specificity of PCR for the IS6110 target obtained 100% in comparison with the culture method. The results confirm the effectiveness of PCR methodology using primers for the IS6110 gene sequence and permit the PCR method to be applied to frozen cutaneous biopsies sent by services that do not identify the M. tuberculosis by the biology molecular method.

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