Establishment of a Mycoplasma hyorhinis challenge model in 5-week-old piglets

Frontiers in Microbiology

Microbiology

József Földi¹  Anna Kollár²  Levente Szeredi²  Miklós Tenk²  Dorottya Földi³  Zsófia Eszter Nagy³  Nikolett Belecz³  Miklós Gyuranecz⁴  Zsuzsa Kreizinger⁵ 

[1] Euvet Bt., Gödöllo, Hungary;National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Budapest, Hungary;Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary;Veterinary Medical Research Institute, Budapest, Hungary;National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Budapest, Hungary;Veterinary Medical Research Institute, Budapest, Hungary;National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Budapest, Hungary;Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary;MolliScience Kft., Biatorbágy, Hungary;Veterinary Medical Research Institute, Budapest, Hungary;National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Budapest, Hungary;MolliScience Kft., Biatorbágy, Hungary;

关键词: challenge;    ELISA;    infection;    Mycoplasma hyorhinis;    PCR;    pig;   

DOI  :  10.3389/fmicb.2023.1209119

 received in 2023-04-20, accepted in 2023-07-17,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionMycoplasma hyorhinis is an emerging swine pathogen with high prevalence worldwide. The main lesions caused are arthritis and polyserositis, and the clinical manifestation of the disease may result in significant economic losses due to decreased weight gain and enhanced medical costs. We aimed to compare two challenge routes to induce M. hyorhinis infection using the same clinical isolate.MethodsFive-week-old, Choice hybrid pigs were inoculated on 2 consecutive days by intravenous route (Group IV-IV) or by intravenous and intraperitoneal routes (Group IV-IP). Mock-infected animals were used as control (control group). After the challenge, the clinical signs were recorded for 28 days, after which the animals were euthanized. Gross pathological and histopathological examinations, PCR detection, isolation, and genotyping of the re-isolated Mycoplasma sp. and culture of bacteria other than Mycoplasma sp. were carried out. The ELISA test was used to detect anti-M. hyorhinis immunoglobulins in the sera of all animals.ResultsPericarditis and polyarthritis were observed in both challenge groups; however, the serositis was more severe in Group IV-IV. Statistically significant differences were detected between the challenged groups and the control group regarding the average daily weight gain, pathological scores, and ELISA titers. Additionally, histopathological scores in Group IV-IV differed significantly from the scores in the control group. All re-isolated strains were the same or a close genetic variant of the original challenge strain.DiscussionOur results indicate that both challenge routes are suitable for modeling the disease. However, due to the evoked more severe pathological lesions and the application being similar to the hypothesized natural route of infection in Group IV-IV, the two-dose intravenous challenge is recommended by the authors to induce serositis and arthritis associated with M. hyorhinis infection.

【 授权许可】

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Copyright © 2023 Földi, Nagy, Belecz, Szeredi, Földi, Kollár, Tenk, Kreizinger and Gyuranecz.