Sentinel Surveillance Reveals Emerging Daptomycin-Resistant ST736 Enterococcus faecium and Multiple Mechanisms of Linezolid Resistance in Enterococci in the United States

Frontiers in Microbiology

Microbiology

Mili Sheth¹  Dhwani Batra¹  Jasmine Padilla²  J. Kristie Johnson³  Christopher A. Elkins⁴  Amy S. Gargis⁴  Davina Campbell⁴  Alison Laufer Halpin⁴  Gillian McAllister⁴  Valerie A. Stevens⁴  Joseph D. Lutgring⁴  Maria Karlsson⁴  J. Kamile Rasheed⁴  Lori M. Spicer⁵  Alyssa G. Kent⁵  Thomas O. Ewing⁵  Wenming Zhu⁶  Valerie Albrecht⁷ 

[1] Biotechnology Core Facility Branch, Division of Scientific Resources, Centers for Disease Control and Prevention, Atlanta, GA, United States;Biotechnology Core Facility Branch, Division of Scientific Resources, Centers for Disease Control and Prevention, Atlanta, GA, United States;ASRT Incorporated, Atlanta, GA, United States;Department of Pathology, University of Maryland School of Medicine, Baltimore, MD, United States;Division of Healthcare Quality Promotion, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, United States;Division of Healthcare Quality Promotion, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, United States;Goldbelt C6, LLC, Chesapeake, VA, United States;Division of Tuberculosis Elimination, National Center for HIV/AIDS, Viral Hepatitis, STD and TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, United States;Office of the Director, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, United States;

关键词: Enterococcus faecalis;    Enterococcus faecium;    daptomycin;    linezolid;    conjugation;    optrA;    transmission;    pheromone responsive plasmid;   

DOI  :  10.3389/fmicb.2021.807398

 received in 2021-11-02, accepted in 2021-12-09,  发布年份 2022

来源: Frontiers

【 摘 要 】

Enterococcus faecalis and faecium with resistance to daptomycin and/or linezolid are emerging globally. We present the genomic characterization of daptomycin- and linezolid-resistant E. faecalis and E. faecium surveillance isolates from the United States, 2013–2016. Daptomycin resistance was low among E. faecalis (2/364, 0.5%) and E. faecium (17/344, 5%). The majority (71%, 12/17) of daptomycin-resistant E. faecium isolates belonged to the emerging ST736 clone and contained mutations in liaFSR and cls previously associated with resistance. However, 1/2 E. faecalis and 3/17 E. faecium did not contain these mutations previously associated with daptomycin resistance. Linezolid resistance was rare among E. faecalis (1/364, 0.3%) and E. faecium (2/344, 0.6%). These two E. faecium isolates, one of which was also resistant to daptomycin and vancomycin, contained the 23S rRNA nucleotide mutation (G2576T) associated with linezolid resistance. Long-read sequencing revealed the linezolid-resistant E. faecalis isolate contained chromosomal- and plasmid-encoded copies of optrA. The chromosomal optrA was located on the recently described Tn6674 multiresistance transposon. The second copy of optrA was encoded on an ∼65 kb mosaic plasmid, with component regions sharing high sequence identity to optrA-encoding multiresistance plasmids of animal origin. The optrA-encoding plasmid contained open reading frames predicted to encode proteins associated with a pheromone-responsive plasmid transfer system, and filter mating experiments confirmed the plasmid was conjugative. Continued surveillance of enterococci is necessary to assess the prevalence and trends of daptomycin and linezolid resistance in the United States, characterize resistance mechanisms and how they transfer, and monitor for emerging sequence types associated with resistance.

【 授权许可】

Unknown   
Copyright © 2022 Gargis, Spicer, Kent, Zhu, Campbell, McAllister, Ewing, Albrecht, Stevens, Sheth, Padilla, Batra, Johnson, Halpin, Rasheed, Elkins, Karlsson and Lutgring.