Isolation and characterization of phosphate-solubilizing bacteria from rhizosphere of poplar on road verge and their antagonistic potential against various phytopathogens

BMC Microbiology

Research Article

Ding Xiaolei¹  Wang Jiangchuan²  Han Xuejiao³  Zeng Qingwei³  Shao Yu³  Tang Lushi³  Muhammad Bilal³  Wu Wanting³  Zhang Yu³ 

[1] Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, 210037, Nanjing, China;School of Accounting, Jiangsu Vocational College of Finance & Economics, 223003, Huaian, China;School of Life Science and Food Engineering, Huaiyin Institute of Technology, 223003, Huaian, China;

关键词: PS activity;    Antagonistic activity;    Biofertilizer;    Biocontrol;    Poplar;   

DOI  :  10.1186/s12866-023-02953-3

 received in 2022-11-14, accepted in 2023-07-19,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundPhosphate-solubilizing bacteria (PSB) can solubilize insoluble phosphate compounds and improve phosphate availability in soil. Road verges are important in urban landscaping, but the population structure of PSB and their ecological functions in the road verge soil is still unclear.ResultsTwenty-one mineral PSB strains and 14 organic PSB strains were isolated from the rhizosphere of poplar on urban road verge. All the mineral PSB strains showed better solubilization to Ca3(PO4)2 than FePO4 or AlPO4. Among them, 7 strains showed high phosphate-solubilizing (PS) activities to Ca3(PO4)2 (150–453 mg/L). All the organic PSB strains displayed weak solubilization to lecithin. 16S rRNA gene-based phylogenetic analysis showed good species diversity of the PSB strains, which belongs to 12 genera: Bacillus, Cedecea, Cellulosimicrobium, Delftia, Ensifer, Paenibacillus, Pantoea, Phyllobacterium, Pseudomonas, Rhizobium, Sinorhizobium and Staphylococcus. Moreover, 8 PSB strains showed various degrees of growth inhibition against 4 plant pathogenic fungi, Fusarium oxysporum S1, F. oxysporum S2, Pythium deliense Meurs Z4, Phomopsis sp. AC1 and a plant pathogenic bacterium, Pectobacterium carotovorum TP1.ConclusionsThe results indicated that these PSB strains could perform multiple ecological functions on road verge. The development and application of bio-agents based on the strains would provide a new strategy for maintaining and improving the ecosystem stability of road verges.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

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