Fungal endophytes as potential biocontrol agent of Panama disease of banana

Egyptian Journal of Biological Pest Control

Research

Jonah Mae F. Taping¹  Maria Elena N. Tanabe¹  Bryan Lloyd P. Bretaña¹  Ma. Teodora N. Cabasan¹  Bernadith T. Borja² 

[1] Department of Biological Sciences, College of Science and Mathematics, University of Southern Mindanao, 9407, Kabacan, Cotabato, Philippines;Division of Plant Pathology, College of Agriculture, University of Southern Mindanao, 9407, Kabacan, Cotabato, Philippines;

关键词: Antagonistic activity;    Biocontrol;    Foc;    Internal transcribed spacer (ITS);    Panama disease of banana;   

DOI  :  10.1186/s41938-023-00727-7

 received in 2023-05-24, accepted in 2023-08-09,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundFusarium odoratissimum (Foc TR4) is a devastating fungal pathogen that causes Panama wilt in Cavendish banana, a high value crop that generates significant revenue. The excessive use of synthetic fungicides for disease control poses risks to both human health and the environment. Consequently, there is a pressing need for eco-friendly alternatives to manage this disease. One potential approach is the utilization of biocontrol agents, which have shown promise in disease control. This study aimed to evaluate the biocontrol potential of fungal endophytes isolated from the naturally-resistant cultivar Cardaba banana against Foc TR4. These fungal isolates were subjected to a dual culture assay, characterized based on morpho-cultural characteristics, and confirmed molecularly using the internal transcribed spacer (ITS) region of rDNA.ResultsOf the 15 fungal endophytes isolated from Cardaba; Blakeslea trispora, Pseudopestalotiopsis theae, Xylaria badia, Nigrospora hainanensis, Colletotrichum gloeosporioides, Xylaria feejeensis, Gymnoascus reessii, Fusarium subglutinans, Rigidoporus vinctus, and Macrophomina phaseolina showed potential antagonistic activity against F. odoratissimum. Isolates X. feejeensis (USMJMTBL10), G. reesii (USMJMTBL11), and M. phaseolina (USMJMTBR1) had the highest antagonistic activity of 87.8–96.6% against F. odoratissimum in vitro.ConclusionThis study presents evidence of the potential of Cardaba-associated endophytes isolated and identified in this research, as effective biocontrol agents against F. odoratissimum, the causal agent of Panama disease in Cavendish banana. Our findings suggest that these endophytes hold promise as a biocontrol agent for managing Foc TR4-infected banana plants. Future research will focus on in planta testing, validation in greenhouse and field trials, as well as development of formulations and application protocols to optimize the utilization of these biocontrol agents in an integrated management approach for Foc TR4-infected bananas.

【 授权许可】

CC BY   
© Egyptian Society for Biological Control of Pests 2023

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