Engineering Saccharomyces cerevisiae for improved biofilm formation and ethanol production in continuous fermentation

Biotechnology for Biofuels and Bioproducts

Research

Qingguo Liu¹  Ting Guo²  Zhenyu Wang³  Di Zhang³  Cheng Wang³  Huifang Zhang³  Xiwei Peng³  Tingqiu Zhou³  Dong Liu³  Huanqing Niu³  Hanjie Ying³  Chenchen Xu³  Yixuan Gao³  Weikai Xu³  Mingwei Zha³  Chenjie Zhu³  Yong Chen³ 

[1]Institute of Industrial Biotechnology, Jiangsu Industrial Technology Research Institute (JITRI), 210032, Nanjing, China

[2]Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, China

[3]State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 211816, Nanjing, China

关键词: Biofilm;    Ethanol;    Continuous fermentation;    Membrane separation;   

DOI  :  10.1186/s13068-023-02356-6

 received in 2023-03-20, accepted in 2023-06-11,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundBiofilm-immobilized continuous fermentation has the potential to enhance cellular environmental tolerance, maintain cell activity and improve production efficiency.ResultsIn this study, different biofilm-forming genes (FLO5, FLO8 and FLO10) were integrated into the genome of S. cerevisiae for overexpression, while FLO5 and FLO10 gave the best results. The biofilm formation of the engineered strains 1308-FLO5 and 1308-FLO10 was improved by 31.3% and 58.7% compared to that of the WT strain, respectively. The counts of cells adhering onto the biofilm carrier were increased. Compared to free-cell fermentation, the average ethanol production of 1308, 1308-FLO5 and 1308-FLO10 was increased by 17.4%, 20.8% and 19.1% in the biofilm-immobilized continuous fermentation, respectively. Due to good adhering ability, the fermentation broth turbidity of 1308-FLO5 and 1308-FLO10 was decreased by 22.3% and 59.1% in the biofilm-immobilized fermentation, respectively. Subsequently, for biofilm-immobilized fermentation coupled with membrane separation, the engineered strain significantly reduced the pollution of cells onto the membrane and the membrane separation flux was increased by 36.3%.ConclusionsIn conclusion, enhanced biofilm-forming capability of S. cerevisiae could offer multiple benefits in ethanol fermentation.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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