Evaluation of ethanol and EDTA concentrations in the expression of biofilm-producing smf-1, rpfF genes in XDR clinical isolates of Stenotrophomonas maltophilia

BMC Microbiology

Research

Fatemeh Fardsanei¹  Amir Peymani¹  Safar Ali Alizadeh¹  Seyed Mahmoud Amin Marashi¹  Farhad Nikkhahi¹  Masoumeh Aslanimehr¹  Amir Javadi¹  Mohadeseh Ostovari Deilamani²  Mehdi Bakht² 

[1]Medical Microbiology Research Center, Qazvin University of Medical Sciences, PO Box: 34199-15315, Qazvin, Iran

[2]Medical Microbiology Research Center, Qazvin University of Medical Sciences, PO Box: 34199-15315, Qazvin, Iran

[3]Student research committee, Qazvin University of Medical Sciences, Qazvin, Iran

关键词: Nosocomial infection;    Biofilm;    Stenotrophomonas maltophilia;    Clinical isolates;    EDTA;    Ethanol;    Checkerboard method;   

DOI  :  10.1186/s12866-023-03008-3

 received in 2023-06-03, accepted in 2023-09-05,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundStenotrophomonas maltophilia is able to cause infections in immunocompromised patients, and the treatment of this opportunistic pathogen is complicated due to its virulence factors, antibiotic resistance, and the ability of the bacteria to produce biofilm. The main goals of this study were to assess the susceptibility of extensively drug-resistant (XDR) isolates to ethanol and EDTA, and evaluating the synergistic effect of these disinfectants, and also survey the effect of exposure to sub-inhibitory concentrations of ethanol and EDTA on the expression of biofilm-producing smf-1, rpfF genes.ResultsThe results showed that EDTA significantly increased the effectiveness of the ethanol and have a synergistic effect. All of the 10 XDR isolates included in the current study harbored smf-1 and rpfF genes and produced biofilm. After exposure to MIC, sub-MIC, synergism, and sub-synergism of ethanol and EDTA, the expression of smf-1 and rpfF genes was repressed significantly.ConclusionIn the current study, it was indicated that the expression of biofilm-producing genes was repressed when bacteria are exposed to different concentrations of ethanol and EDTA. Future studies should include more complex microbial communities residing in the hospitals, and more disinfectants use in hospitals. Expression of other virulence genes in different conditions is suggested.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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