Detection of micro RNA hsa-let-7e in peripheral blood mononuclear cells infected with dengue virus serotype-2: preliminary study

1st International Conference on Tropical Medicine and Infectious Diseases (ICTROMI) Faculty of Medicine Universitas Sumatera Utara

Masyeni, S.^1 ; Hadi, U.^2 ; Kuntaman^2 ; Yohan, B.^3 ; Margyaningsih, N.I.^3 ; Sasmono, R.T.^3

Faculty of Medicine and Health Science, University of Warmadewa, JlTerompong 24, Denpasar-Bali, Indonesia^1

Faculty of Medicine, University of Airlangga, Mayjen Prof. Dr. Moestopo 47, Pacar Kembang, Surabaya, Kota SBY, Jawa Timur, Indonesia^2

Eijkman Institute for Molecular Biology, Jl Diponegoro no 69, Jakarta, Indonesia^3

关键词: Clinical isolates;    Dengue virus;    Healthy volunteers;    Peripheral blood mononuclear cells;    Plasma leakage;    Pro-inflammatory cytokines;    Quantitative real time PCR;    Suppressor of cytokine signaling 3;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/125/1/012010/pdf
DOI  :  10.1088/1755-1315/125/1/012010

来源: IOP

【 摘 要 】

Pathogenesis of dengue infection is still obscure. Recently, the role of microRNA has been associated with the cytokine storm which leads to plasma leakage in endothelial cells. The objective of our study was to determine whether particular microRNA is overexpressed in PBMCs infected with DENV and to assess its correlation to the expression of suppressor of cytokine signaling 3 (SOCS3) proteins to increase the production of pro-inflammatory cytokines. We report the result of a preliminary study on the expression of microRNA hsa-let-7e. The peripheral blood mononuclear cells (PBMCs) from the healthy volunteer were infected with the clinical isolate of DENV-2. RNA was extracted with miRCURYLNATMExiqon. Quantitative Real-Time PCR was used to measure the relative expression of hsa-let-7e micro RNA and the mRNA of SOCS3 proteins. MicroRNA hsa-let-7e expression was increased in PBMCs upon DENV-2 infection. The relative expression of hsa-let-7e is detected at 1.46 folds relative to uninfected PBMCs in 4 hours post-infection and decreased in 19 hours post infection. In contrast, the expression of mRNA of SOCS3 was inversely expressed with hsa-let-7 expression. MicroRNA was overexpressed in PBMCs upon infection with DENV-2. This microRNA may bind the SOCS3 and contribute to the pathogenesis of dengue infection.