期刊论文详细信息
FEBS Letters
Calcium uptake by tonoplast and plasma membrane vesicles from spinach leaves
Greppin, H.1  Penel, C.1  Malatialy, L.1 
[1] Laboratoire de Physiologie Végétale, Université de Genève, 3, place de l'Université, 1211 Genève 4, Switzerland
关键词: Free-flow electrophoresis;    Membrane separation;    Calmodulin;    Verapamil;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    EGTA;    ethylene glycol bis(aminoethylether) N;    N′-tetraacetic acid;   
DOI  :  10.1016/0014-5793(88)81383-8
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

Tonoplast- and plasma membrane-enriched fractions were simultaneously prepared from the same crude membrane suspension from spinach leaves by free-flow electrophoresis. They were used for in vitro ATP-dependent Ca2+ -uptake studies. The plasma membrane-enriched fraction accumulated Ca2+ at a rather slow rate by a mechanism which was inhibited by orthovanadate, insensitive to nitrate and activated by calmodulin. The tonoplast-enriched fraction accumulated Ca2+ at a high rate for 5 min, then the accumulated Ca2+ progressively leaked out of the vesicles. The tonoplast transport system was partly inhibited by nitrate, but also by vanadate, completely abolished by the protonophore CCCP and activated by calmodulin and the Ca2+ antagonist verapamil.

【 授权许可】

Unknown   

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