Calcium uptake by tonoplast and plasma membrane vesicles from spinach leaves

FEBS Letters

Greppin, H.¹  Penel, C.¹  Malatialy, L.¹ 

[1]Laboratoire de Physiologie Végétale, Université de Genève, 3, place de l'Université, 1211 Genève 4, Switzerland

关键词: Free-flow electrophoresis;    Membrane separation;    Calmodulin;    Verapamil;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    EGTA;    ethylene glycol bis(aminoethylether) N;    N′-tetraacetic acid;   

DOI  :  10.1016/0014-5793(88)81383-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tonoplast- and plasma membrane-enriched fractions were simultaneously prepared from the same crude membrane suspension from spinach leaves by free-flow electrophoresis. They were used for in vitro ATP-dependent Ca²⁺ -uptake studies. The plasma membrane-enriched fraction accumulated Ca²⁺ at a rather slow rate by a mechanism which was inhibited by orthovanadate, insensitive to nitrate and activated by calmodulin. The tonoplast-enriched fraction accumulated Ca²⁺ at a high rate for 5 min, then the accumulated Ca²⁺ progressively leaked out of the vesicles. The tonoplast transport system was partly inhibited by nitrate, but also by vanadate, completely abolished by the protonophore CCCP and activated by calmodulin and the Ca²⁺ antagonist verapamil.

【 授权许可】

Unknown