Molecular characterization and antifungal activity of lipopeptides produced from Bacillus subtilis against plant fungal pathogen Alternaria alternata

BMC Microbiology

Research

K. S. Tharun Kumar¹  V. Kavan Kumar²  B. N. Ramesh³  K. S. Shiva Kumar⁴  S. Shyamalamma⁵  S. N. Pavan⁵  H. C. Girish⁵  M. S. Nagaraj⁵  S. N. Nagesha⁵  B. N. Harish⁵  C. Pradeep⁵ 

[1] Bangalore City College, 560043, Bangalore, India;College of Technology and Engineering, MPUAT, 313001, Udaipur, Rajasthan, India;ICAR-AICRP on Post Harvest Technology, UAS, GKVK, 560065, Bengaluru, India;Indian Academy College, 560043, Bangalore, India;University of Agricultural Sciences, 560065, Bangalore, India;

关键词: B. subtilis;    Lipopeptides;    HPLC;    Antifungal activity;    Alternaria alternata;   

DOI  :  10.1186/s12866-023-02922-w

 received in 2023-02-13, accepted in 2023-06-28,  发布年份 2023

来源: Springer

【 摘 要 】

Over 380 host plant species have been known to develop leaf spots as a result of the fungus Alternaria alternata. It is an aspiring pathogen that affects a variety of hosts and causes rots, blights, and leaf spots on different plant sections. In this investigation, the lipopeptides from the B. subtilis strains T3, T4, T5, and T6 were evaluated for their antifungal activities. In the genomic DNA, iturin, surfactin, and fengycin genes were found recovered from B. subtilis bacterium by PCR amplification. From different B. subtilis strains, antifungal Lipopeptides were extracted, identified by HPLC, and quantified with values for T3 (24 g/ml), T4 (32 g/ml), T5 (28 g/ml), and T6 (18 g/ml). To test the antifungal activity, the isolated lipopeptides from the B. subtilis T3, T4, T5, and T6 strains were applied to Alternaria alternata at a concentration of 10 g/ml. Lipopeptides were found to suppress Alternaria alternata at rates of T3 (75.14%), T4 (75.93%), T5 (80.40%), and T6 (85.88%). The T6 strain outperformed the other three by having the highest antifungal activity against Alternaria alternata (85.88%).

【 授权许可】

CC BY   
© The Author(s) 2023

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