MSCs’ conditioned media cytokine and growth factor profiles and their impact on macrophage polarization

Stem Cell Research & Therapy

Research

Irina I. Vlasova¹  Shakir Suleimanov²  Nastasia Kosheleva³  Alexander Korneev⁴  Andrey Svistunov⁵  Peter Timashev⁶  Maria Peshkova⁶ 

[1] Institute for Regenerative Medicine, Sechenov University, 119991, Moscow, Russia;Institute for Regenerative Medicine, Sechenov University, 119991, Moscow, Russia;Laboratory of Clinical Smart Nanotechnologies, Sechenov University, 119991, Moscow, Russia;Institute for Regenerative Medicine, Sechenov University, 119991, Moscow, Russia;Laboratory of Clinical Smart Nanotechnologies, Sechenov University, 119991, Moscow, Russia;FSBSI Institute of General Pathology and Pathophysiology, 125315, Moscow, Russia;Institute for Regenerative Medicine, Sechenov University, 119991, Moscow, Russia;Laboratory of Clinical Smart Nanotechnologies, Sechenov University, 119991, Moscow, Russia;Laboratory of the Polymers Synthesis for Medical Applications, Sechenov University, 119991, Moscow, Russia;Sechenov First Moscow State Medical University, 119991, Moscow, Russia;World-Class Research Center “Digital Biodesign and Personalized Healthcare”, Sechenov University, 119991, Moscow, Russia;Institute for Regenerative Medicine, Sechenov University, 119991, Moscow, Russia;Laboratory of Clinical Smart Nanotechnologies, Sechenov University, 119991, Moscow, Russia;

关键词: Mesenchymal stromal cells;    Conditioned media;    Secretome;    Cytokines;    Immunomodulation;    Macrophages;   

DOI  :  10.1186/s13287-023-03381-w

 received in 2022-10-20, accepted in 2023-05-18,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThere is a growing body of evidence that multipotent mesenchymal stromal cells’ (MSCs’) remarkable therapeutic potential is attributed not only to their differentiation and regenerative capacity, but also to the paracrine effect, underlying their immunomodulatory properties. MSCs’ secretome (i.e., cytokines, growth factors, and extracellular vesicles) is therefore increasingly discussed in the context of their ability to modulate inflammatory response and promote regeneration. There is evidence that 2D or 3D culturing conditions have an impact on the cells’ secretome, and here we aimed to compare the secretion of cytokines and growth factors in human MSCs from different sources cultured in 2D and 3D conditions and assess their effect on human macrophages polarization in vitro.MethodsMSCs were derived from human adipose tissue, bone marrow, gingiva, placenta, and umbilical cord, cultured as monolayers or as cell spheroids. Their cytokine profiles were analyzed, and data standardization was carried out using a z-score. Human peripheral blood mononuclear cells-derived macrophages were then treated with umbilical cord-derived MSCs’ conditioned media and their effect on macrophages polarization was assessed.ResultsOur findings suggest that umbilical cord-derived MSCs’ conditioned media demonstrated the highest cytokine and growth factor levels and despite mostly pro-inflammatory cytokine profile were able to promote anti-inflammatory macrophage polarization.ConclusionsUmbilical cord-derived MSCs’ conditioned media hold great potential for therapeutic use, demonstrating significant anti-inflammatory effect on human macrophages.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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Fig. 3