A comparative study on normal and obese mice indicates that the secretome of mesenchymal stromal cells is influenced by tissue environment and physiopathological conditions

Cell Communication and Signaling

Umberto Galderisi¹  Domenico Aprile²  Giovanni Di Bernardo²  Nicola Alessio²  Serife Ayaz-Guner³  Servet Özcan⁴  Mustafa B. Acar⁴  Gianfranco Peluso⁵ 

[1] CNR;Department of Experimental Medicine, Luigi Vanvitelli Campania University;Department of Molecular Biology and Genetics, Faculty of Life and Natural Science, Abdullah Gül University;Genome and Stem Cell Center (GENKOK), Erciyes University;Research Institute on Ecosystems (IRET);

关键词: Obesity;    Mesenchymal stromal cells;    Secretome;   

DOI  :  10.1186/s12964-020-00614-w

来源: DOAJ

【 摘 要 】

Abstract Background The term mesenchymal stromal cells (MSCs) designates an assorted cell population comprised of stem cells, progenitor cells, fibroblasts, and stromal cells. MSCs contribute to the homeostatic maintenance of many organs through paracrine and long-distance signaling. Tissue environment, in both physiological and pathological conditions, may affect the intercellular communication of MSCs. Methods We performed a secretome analysis of MSCs isolated from subcutaneous adipose tissue (sWAT) and visceral adipose tissue (vWAT), and from bone marrow (BM), of normal and obese mice. Results The MSCs isolated from tissues of healthy mice share a common core of released factors: components of cytoskeletal and extracellular structures; regulators of basic cellular functions, such as protein synthesis and degradation; modulators of endoplasmic reticulum stress; and counteracting oxidative stress. It can be hypothesized that MSC secretome beneficially affects target cells by the horizontal transfer of many released factors. Each type of MSC may exert specific signaling functions, which could be determined by looking at the many factors that are exclusively released from every MSC type. The vWAT-MSCs release factors that play a role in detoxification activity in response to toxic substances and drugs. The sWAT-MSC secretome contains proteins involved in in chondrogenesis, osteogenesis, and angiogenesis. Analysis of BM-MSC secretome revealed that these cells exert a signaling function by remodeling extracellular matrix structures, such as those containing glycosaminoglycans. Obesity status profoundly modified the secretome content of MSCs, impairing the above-described activity and promoting the release of inflammatory factors. Conclusion We demonstrated that the content of MSC secretomes depends on tissue microenvironment and that pathological condition may profoundly alter its composition. Video abstract

【 授权许可】

Unknown