Increased clonal dissemination of OXA-232-producing ST15 Klebsiella pneumoniae in Zhejiang, China from 2018 to 2021

Infectious Diseases of Poverty

Research Article

Chang Cai¹  Ling Huang²  Lingbin Shu³  Hongwei Zhou³  Rong Zhang³  Congcong Liu³  Yonglu Huang³  Yanyan Zhang³  Qiaoling Sun³  Sheng Chen⁴  Xuemei Yang⁴  Xiaoyan Wu⁵ 

[1] College of Animal Science and Technology, Zhejiang Agricultural and Forestry University, Hangzhou, China;Department of Clinical Laboratory Medicine, Maternal and Child Health Hospital of Yuhang District, Hangzhou, China;Department of Clinical Laboratory, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China;Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong, China;The Clinical Laboratory, Jiaxing Second Hospital, Jiaxing, China;

关键词: Klebsiella pneumoniae;    Escherichia coli;    OXA-232;    ColKP3;    Clonal dissemination;   

DOI  :  10.1186/s40249-023-01051-w

 received in 2022-09-02, accepted in 2023-01-03,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundOXA-232-producing Klebsiella pneumoniae was first identified in China in 2016, and its clonal transmission was reported in 2019. However, there are no prevalence and genotypic surveillance data available for OXA-232 in China. Therefore, we investigated the trends and characteristics of OXA-232 type carbapenemase in Zhejiang Province, China from 2018 to 2021.MethodsA total of 3278 samples from 1666 patients in the intensive care units were collected from hospitals in Zhejiang Province from 2018 to 2021. Carbapenem-resistant isolates were initially selected by China Blue agar plates supplemented with 0.3 μg/ml meropenem, and further analyzed by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry identification, immune colloidal gold technique, conjugation experiment, antimicrobial susceptibility testing and whole genome sequencing.ResultsA total of 79 OXA-producing strains were recovered, with the prevalence increased from 1.8% [95% confidence interval (CI): 0.7–3.7%] in 2018 to 6.0% (95% CI: 4.4–7.9%) in 2021. Seventy-eight strains produced OXA-232 and one produced OXA-181. The blaOXA-232 gene in all strains was located in a 6141-bp ColKP3-type non-conjugative plasmid and the blaOXA-181 gene was located in a 51,391-bp ColKP3/IncX3-type non-conjugative plasmid. The blaOXA-232-producing K. pneumoniae was dominated (75/76) by isolates of sequence type 15 (ST15) that differed by less than 80 SNPs. All OXA-producing strains (100%, 95% CI: 95.4–100.0%) were multidrug-resistant.ConclusionsFrom 2018 to 2021, OXA-232 is the most prevalent OXA-48-like derivative in Zhejiang Province, and ST15 K. pneumoniae isolates belonging to the same clone are the major carriers. The transmission of ColKP3-type plasmid to E. coli highlighted that understanding the transmission mechanism is of great importance to delay or arrest the propagation of OXA-232 to other species.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

41116_2023_36_Article_IEq258.gif

41116_2023_36_Article_IEq257.gif

41116_2023_36_Article_IEq255.gif

41116_2023_36_Article_IEq246.gif

41116_2023_36_Article_IEq205.gif