Emergence and genomics of OXA-232-producing Klebsiella pneumoniae in a hospital in Yancheng, China

Journal of Global Antimicrobial Resistance

Hong Du¹  Jinnan Lv²  Zhichen Zhu²  Haifeng Huang³  Linlin Xu³  Ya Xu³  Chunlei Shi³  Liang Chen⁴  Yumei Xu⁵  Min Wang⁶  Ruifu Yang⁶ 

[1] Hackensack Meridian School of Medicine at Seton Hall University, Nutley, NJ 07110, USA;Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China;Department of Laboratory Medicine, The First People's Hospital of Yancheng City, Yancheng, Jiangsu, China;Hackensack-Meridian Health Center for Discovery and Innovation, Nutley, NJ 07110, USA;Laboratory Department, Shanghai Xuhui Central Hospital, Shanghai, China;State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China;

关键词: Klebsiella pneumoniae;    OXA-232;    Clonal dissemination;    Genetic elements;    ST15;   

DOI  :  

来源: DOAJ

【 摘 要 】

ABSTRACT:Objectives: The aims of this study were to infer the phylogenetic relationship of OXA-232-producing Klebsiella pneumoniae (OXA232Kp) strains collected from a Chinese hospital and to determine the composition and genetic background of antimicrobial resistance genes (ARGs) among these strains. Methods: Three non-duplicate OXA232Kp strains were collected from a Chinese hospital. Whole-genome sequencing was used to determine their genome sequences and then a genomic comparison of ARG-carrying genetic elements from the three strains with related sequences was performed. Phylogenetic analysis was conducted by constructing a maximum-likelihood phylogenetic tree. Results: Compared with other Chinese sequence type 15 (ST15)-OXA232Kp strains, the three ST15-OXA232Kp strains in this study could be divided into a single subgroup in phylogenetic relationship. The composition and genetic background of ARGs were identical in the three strains. Three ARG-carrying genetic elements or multidrug resistance (MDR) regions were determined, including a truncated Tn2013-like IS-based transposition unit, a unit transposition Tn6867b and a 40.9-kb MDR region. Conclusion: This study reported clonal dissemination of ST15-OXA232Kp strains carrying multiple ARGs in a Chinese hospital. A comprehensive evolutionary and genomics analysis provided a deeper understanding of OXA232Kp. Further surveillance and study should be advocated to prevent the dissemination of OXA232Kp strains in China.

【 授权许可】

Unknown