Effects of Astragalus polysaccharide on immune function in B16 melanoma mice

TMR Integrative Medicine

article

Fang-Hua Wu¹  Kui Chen¹  De-Kun Zhang¹  Chao Gong¹  Jiang-Yue Yu¹  Shen-Zhou Huang¹  Chen-Xin Huang¹  Li-Qun Wang¹ 

[1] Department ofGeneral Surgery, Fujian Medical UniversityAffiliated Fuzhou First Hospital

关键词: Melanoma;    Astragalus polysaccharide;    Tumor;    Immune function;   

DOI  :  10.12032/TMRIM202105012

学科分类：医学（综合）

来源: TMR publishing group

【 摘 要 】

Objective: To examine the effects of Astragalus polysaccharide on immune function in B16 melanoma mice. Method: Forty male C57BL/6 mice were divided equally into a control group, model group, Astragaluspolysaccharide low-dose group, and Astragalus polysaccharide high-dose group, with 10 mice per group. B16 cellswere used to develop a mouse model of melanoma. After B16 engraftment, 40 mg/kg and 80 mg/kg Astragaluspolysaccharide was administered by gavage every day to the Astragalus polysaccharide low-dose group andAstragalus polysaccharide high-dose group, respectively. Splenic index, thymic index, tumor growth curves, andtumor inhibition rates were measured. Flow cytometry was used to measure proportions of peripheral blood Tlymphocyte subsets. Hematoxylin and eosin staining was used to examine histopathological changes in tumors. Immunofluorescence double staining was used to identify myeloid-derived suppressor cells in tumor tissues. Results: In the Astragalus polysaccharide high-dose group, splenic and thymic indices were significantly increasedand tumor growth was inhibited in melanoma mice. Flow cytometry demonstrated increased CD4+ and CD4+/CD8+ T-cell ratios in the high-dose Astragalus polysaccharide group. HE staining demonstrated significantly decreasednumbers of tumor cells among mice with melanoma in the high-dose Astragalus polysaccharide group. Immunofluorescence double staining demonstrated significantly decreased numbers of myeloid-derived suppressorcells in tumor tissues in the high-dose Astragalus polysaccharide group. Conclusion: Astragalus polysaccharideinhibits tumor growth, increases splenic and thymic indices, increases CD4+ and CD4+/CD8+ T-cell ratios, anddecreases myeloid-derived suppressor cell numbers in melanoma mice. Our results indicate Astragaluspolysaccharide may enhance immune function resulting in inhibition of tumorigenesis and tumor progression.

【 授权许可】

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