Bacteriostatic and Haemolytic Activities of Extracts and Compounds of Commiphora swynnertonii

Research Journal of Pharmacognosy

article

Zaituni Msengwa¹  David Credo¹  Magesa Mafuru⁴  James Mwesongo⁵  Faith Philemon Mabiki¹  Beda John Mwang’onde⁵  Madundo Mkumbukwa Mtambo⁶  Lughano Jeremy Kusiluka⁷  Robinson HammerthonMdegela⁸  John Elmerdahl Olsen² 

[1] Department of Chemistry and Physics, Sokoine University of Agriculture;Department of Veterinary and Animal Sciences, University of Copenhagen;Department of Biological and Pre-clinical Studies, Institute of Traditional Medicine, Muhimbili University of Health and Allied Sciences;Department of Clinical Pharmacology and Therapeutics, Hubert Kairuki Memorial University;Department of Biosciences, Sokoine University of Agriculture;Tanzania Industrial Research and Development Organisation;Department of Microbiology and Parasitology, School of Medicine and Dentistry, University of Dodoma;Department of Veterinary Medicine and Public Health, Sokoine University of Agriculture

关键词: antibacterial;    Commiphora swynnertonii;    Medicinal plant;    Red blood cells;   

DOI  :  10.22127/rjp.2023.370231.2009

学科分类：环境科学（综合）

来源: Iranian Society of Pharmacognosy

【 摘 要 】

Background and objective: Commiphora swynnertonii (Pax) is used in traditional medicine to treatinfectious diseases. Previous studies have reported antimicrobial activity of this plant; however, theactivity of compounds that are present in extracts of this plant has not been thoroughly documented.Likewise, the primary mode of action (bactericidal or bacteriostatic) and the possible toxicity on redblood cells have not been reported. Methods: Extracts of leaves, whole root, root bark, root wood,whole stem, stem bark and stem wood, were produced using hexane, dichloromethane, methanol andwater. Cold and hot extraction methods were employed. Antibacterial activity of extracts was testedagainst selected medically important Gram-positive and Gram-negative bacteria by growth inhibition,minimum inhibitory concentrations and time kill assays. Moreover, haemolytic activity against sheepred blood cells was determined in vitro. Results: The hexane extracts of whole root and root bark,methanol extracts of root wood, and dichloromethane extracts from the leaves of C. swynnertoniiinhibited the growth of S. aureus. MIC values for the extracts and compounds, indicated moderateactivity against Gram-positive bacteria (Staphylococcus aureus, Streptococci species and Enterococcispecies) while the activity against Gram-negative bacteria (Escherichia coli, Klebsiella species,Pseudomonas aeruginosa, Salmonella species, Shigella sonnei and Yersinia enterocolitica) was weak.Time kill profiles showed the extracts have bacteriostatic activity against S. aureus, and lowhaemolytic effect, except for extracts of whole root and leaves at the concentration of 1000 µg/mL.Conclusion: Extracts of C. swynnertonii showed bacteriostatic activity against Gram-positive bacteriawith low toxicity on red blood cells.

【 授权许可】

CC BY-NC