Trypanocidal activity of ethanolic extracts of Commiphora swynnertonii Burtt on Trypanosoma congolense

BMC Complementary and Alternative Medicine

Research Article

Richard S. Silayo¹  Yakob P. Nagagi²  Eliningaya J. Kweka³ 

[1] Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, P. O. Box 3019, CHUO KIKUU, Morogoro, Tanzania;Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, P. O. Box 3019, CHUO KIKUU, Morogoro, Tanzania;Division of Livestock and Human Diseases Vector Control, Tropical Pesticides Research Institute, P.O. Box 3024, Arusha, Tanzania;Division of Livestock and Human Diseases Vector Control, Tropical Pesticides Research Institute, P.O. Box 3024, Arusha, Tanzania;Department of Medical Parasitology and Entomology, Catholic University of Health and Allied Sciences, P.O. Box 1464, Mwanza, Tanzania;

关键词: Trypanocidal activity;    Commiphora swynnertonii;    Trypanosoma congolense;   

DOI  :  10.1186/s12906-016-1191-0

 received in 2016-02-24, accepted in 2016-07-05,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundAfrican trypanosomosis is the disease caused by extracellular protozoan parasites of the genus Trypanosoma transmitted by tsetse flies. The current study has evaluated the trypanocidal activity of Commiphora swynnertonii extracts on Trypanosoma congolense.MethodsThe effect of ethanolic stem bark and resinous extracts on motility of T. congolense was evaluated by in vitro study at concentrations of 2 mg/ml and 4 mg/ml. Then, trypanocidal activity was evaluated by drug incubation infectivity test using mice at concentrations of 0.4 mg/ml and 2 mg/ml. In both studies negative (without drug) and positive (diminazene diaceturate) controls were used.ResultsThe in vitro study showed that, ethanolic stem bark extract of C. swynnertonii at concentration of 4 mg/ml caused complete cessation of motility for T. congolense in 30 min. However, resinous ethanolic extract had delayed effect on cessation of motility of T. congolense observed at 90 and 100 min post-incubation at concentrations of 4 mg/ml and 2 mg/ml respectively. The drug incubation infectivity test study depicted that ethanolic stem bark extract at concentration of 2 mg/ml significantly (p = 0.000) reduced the infectivity of T. congolense in mice. However, it did not vary significantly (P =0.897) with group treated with diminazene diaceturate incubated mixture.ConclusionThe current study has provided evidence that, ethanolic stem bark extract of C. swynnertonii possess trypanocidal activity against T. congolense. Based on these findings, further studies are recommended to determine its potential as a lead to trypanocidal drug discovery.

【 授权许可】

CC BY   
© The Author(s). 2016